Improving longitudinal resolution of Airy beams two-photon volume imaging with fluorescence lifetime imaging

PLUTO / PLUTO-2 Spatial Light Modulators
Bessel-/ Airy Beam Generation Microscopy
Published on:
Authors: Guo, Yong; Wang, Luwei; Zhu, Yinru; Gao, Xinwei; Weng, Xiaoyu; Liu, Jinyuan; Yan, Wei & Qu, Junle
Abstract:
“The non-diffracting Airy beams have been reported to increase acquisition speed by projecting the axially significantly elongated information to a two-dimensional (2D) image. Due to the elongated focal length, an Airy volumetric image can cover information as much as stack images with multiple Gaussian images at different imaging depths, which significantly increases the volumetric imaging rate. However, the projection of three-dimensional (3D) structures on a 2D plane is usually indistinguishable, especially those that overlap along the axis. Therefore, we proposed an axially resolved volume imaging technique based on a two-photon fluorescence lifetime microscopy imaging (FLIM) by using Airy beams.”

Restricted Access

Publication: Optics Communications
Issue/Year: Optics Communications, Volume 530; Pages 129151; 2023
DOI: 10.1016/j.optcom.2022.129151
Link: https://doi.org/10.1016/j.optcom.2022.129151

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