Efficient generation of high-contrast Bessel light sheets: a flexible method for improved imaging

PLUTO / PLUTO-2 Spatial Light Modulators
Beam Shaping / Beam Steering Bessel-/ Airy Beam Generation Imaging/ Image Processing Microscopy
Published on:
Authors: Jixiang Wang, Xin Xu, Hong Ye, Xin Zhang, and Guohua Shi
Abstract:

Light-sheet fluorescence microscopy plays a pivotal role in the field of biological 3D imaging. Among its various implementations, non-diffracting light sheets have garnered significant attention due to their remarkable ability to achieve a favorable balance between field of view and resolution. However, the presence of noticeable side-lobe effects in the non-diffracting light sheets poses challenges, including decreased contrast and an increased risk of phototoxicity. While amplitude modulation-based methods effectively suppress side-lobe influences, their transmission efficiency remains suboptimal. To address these limitations, this article introduces an approach based on phase modulation, facilitating the convenient and flexible generation of light sheets that effectively suppress side-lobe effects while maintaining high transmission efficiency. Importantly, our method enables rapid determination of optimal phase parameters, successfully suppressing the peak intensity of the first and second side lobes to levels exceeding 98% and 99%, respectively. Subsequently, experimental results substantiate the light sheet’s exceptional contrast-enhancing capabilities.

Open Access

Publication: Optics Express
Issue/Year: Opt. Express 32, 25247-25260 (2024)
DOI: 10.1364/OE.528127
Link: https://doi.org/10.1364/OE.528127

Related Papers

LETO / LETO-3 Spatial Light Modulators
Authors: Xin Zhang, Yanwen Hu, Haolin Lin, Hao Yin, Zhen Li, Shenhe Fu and Zhenqiang Chen

Flat lens–based subwavelength focusing and scanning enabled by Fourier translation

Applications: Beam Shaping / Beam Steering,Pulse Application / -Shaping
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Qu, Z., Wang, K., Feng, C., Wu, T., Zhang, Y., Zhao, X., & Wang, J. L.

Optofluidic sorting of microparticles using Airy beams

Applications: Beam Shaping / Beam Steering,Bessel-/ Airy Beam Generation,Optical Trapping /-Tweezers
LC 2012 PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Jiaosheng Li, Yifei Chen, Tianyun Liu, Bo Wu & Qinnan Zhang

Single-pixel Fresnel incoherent correlation holography compressed imaging using a Trumpet network

Applications: Deep Learning / Neuronal Network,Digital-/ Computer Holography/ CGH,Imaging/ Image Processing
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Juntao Hu, Wenjun Wei, Xinzhong Li, and Yixian Qian

Generation of polygonal non-diffracting beams via angular spectral phases

Applications: Beam Shaping / Beam Steering,Higher Order Modes / Optical Vortex / OAM,Microscopy
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:M. P. Morales Rodríguez, E. García Herrera, O. Magaña Loaiza, B. Perez-Garcia, F. Marroquín Gutíerrez, B. M. Rodríguez-Lara

Spatial light mode analogues of generalized quantum coherent states

Applications: Beam Shaping / Beam Steering,Higher Order Modes / Optical Vortex / OAM
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Kyriakos Skarsoulis, Konstantinos Makris, Christophe Moser, and Demetri Psaltis

Ptychographic imaging with a fiber endoscope via wavelength scanning

Applications: Deep Learning / Neuronal Network,Imaging/ Image Processing
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Wangting Zhou, Hui Xie, Kezhou Li, Zhiyuan Sun, Jiangshan He, Zhen Yuan, Xunbin Wei, Xueli Chen

Flexible depth-of-focus, depth-invariant resolution photoacoustic microscopy with Airy beam

Applications: Bessel-/ Airy Beam Generation,Microscopy,Pulse Application / -Shaping
LETO / LETO-3 Spatial Light Modulators
Authors:Manuel Hüpfel and Gerd Ulrich Nienhaus

Beam shaping in light-sheet microscopy: an experimental analysis

Applications: Beam Shaping / Beam Steering,Microscopy
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Ethan Tseng, Grace Kuo, Seung-Hwan Baek, Nathan Matsuda, Andrew Maimone, Florian Schiffers, Praneeth Chakravarthula, Qiang Fu, Wolfgang Heidrich, Douglas Lanman & Felix Heide

Neural étendue expander for ultra-wide-angle high-fidelity holographic display

Applications: AR/VR/MR / Holographic Display,Imaging/ Image Processing
GAEA / GAEA-2 Spatial Light Modulators
Authors:Wenxiang Yan, Yuan Gao, Zheng Yuan, Xian Long, Zhaozhong Chen, Zhi-Cheng Ren, Xi-Lin Wang, Jianping Ding, and Hui-Tian Wang

Energy-flow-reversing dynamics in vortex beams: OAM-independent propagation and enhanced resilience

Applications: Beam Shaping / Beam Steering,Higher Order Modes / Optical Vortex / OAM
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Siyuan Wang, Zonglin Guo, Yu Zhao, Yanbo Pei, Hongyan Shi, Jingbo Zhang, Jian Wang

Deep field-of-view and passive tomography based on Airy light field

Applications: Bessel-/ Airy Beam Generation,Imaging/ Image Processing
LETO / LETO-3 Spatial Light Modulators
Authors:Jian He, Jiahao Chen, Yimin Zhou, Fei Wang, Yangjian Cai, and Guoquan Zhou

Realization of double uniform line self-focusing of elliptical Airyprime beams

Applications: Beam Shaping / Beam Steering,Bessel-/ Airy Beam Generation
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Zheng, Junpeng and Zhao, Ruhao and Zhang, Cong and Xiang, Meng and Zhou, Gai and Xu, Yi and Fu, Songnian and Qin, Yuwen

Tailoring the Generalized 2D Airy Beam

Applications: Bessel-/ Airy Beam Generation
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Shuo Liu, Yi Zheng, Huiming Xiao, Shengxiang Shan, Xuejuan Liu, Sohail Ahmad, Anwar Manzoor Rana, Shubo Cheng, Wenxing Yang, Shaohua Tao

Generation of a controllable multi-spiral beam by using the modulated helico-conical phases

Applications: Beam Shaping / Beam Steering,Higher Order Modes / Optical Vortex / OAM,Optical Trapping /-Tweezers
GAEA / GAEA-2 Spatial Light Modulators
Authors:Chen J, Yang C, Wang D, Wang K.

Optical Imaging Method of Synthetic-Aperture Radar for Moving Targets

Applications: Adaptive Optics / Wavefront Control,Beam Shaping / Beam Steering
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Zhuo Sun, Juntao Hu, Yishu Wang, Xinzhong Li, and Yixian Qian

Generation for high-dimensional caustics and artificially tailored structured caustic beams

Applications: Beam Shaping / Beam Steering
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Rui Xiong, Xiangchao Zhang, Xinyang Ma, Leheng Li, Zhanghao Ni, and Xiangqian Jiang

Adaptive coded phase mask design and high-quality image reconstruction for interference-less coded aperture correlation holography

Applications: Deep Learning / Neuronal Network,Digital-/ Computer Holography/ CGH,Imaging/ Image Processing
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:C. Lutz, S. Schwarz, J. Marx, C. Esen, R. Hellmann

Combination of an axicon and a spatial light modulator for the generation of multi-Bessel beams for microdrilling using ultrashort pulsed laser

Applications: Bessel-/ Airy Beam Generation,Materials Processing / Optical Fabrication
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Patrick Cameron, Baptiste Courme, Daniele Faccio, Hugo Defienne

Tutorial: Shaping the Spatial Correlations of Entangled Photon Pairs

Applications: Beam Shaping / Beam Steering,Imaging/ Image Processing,Optical Computing / Quantum Optics
GAEA / GAEA-2 Spatial Light Modulators
Authors: Zhongzheng Lin, Weihang Zhong, Lixun Wu, Lin He, Hongjia Chen, Jianqi Hu, Yujie Chen, Siyuan Yu

Azimuthal beam shaping in orbital angular momentum basis

Applications: Beam Shaping / Beam Steering,Higher Order Modes / Optical Vortex / OAM
PLUTO / PLUTO-2 Spatial Light Modulators
Authors: Yongqiang Chen, Chenggui Luo, Shiqi Wang, Yanping Li, Binglin Shen, Rui Hu, Junle Qu, Liwei Liu

Rapid, high-contrast, and steady volumetric imaging with Bessel-beam-based two-photon fluorescence microscopy

Applications: Microscopy
LETO / LETO-3 Spatial Light Modulators
Authors:Jian He, Jiahao Chen, Yimin Zhou, Yiqing Xu, Yongzhou Ni, Fei Wang, Yangjian Cai, and Guoquan Zhou

Realization of a circularly transformed Airyprime beam with powerful autofocusing ability

Applications: Bessel-/ Airy Beam Generation,Higher Order Modes / Optical Vortex / OAM
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Claudio Conci, Laura Sironi, Emanuela Jacchetti, Davide Panzeri, Donato Inverso, Rebeca Martínez Vázquez, Roberto Osellame, Maddalena Collini, Giulio Cerullo, Giuseppe Chirico, Manuela Teresa Raimondi

In vivo label-free tissue histology through a microstructured imaging window

Applications: Beam Shaping / Beam Steering,Digital-/ Computer Holography/ CGH
PLUTO / PLUTO-2 Spatial Light Modulators
Authors: Sebastian Cremaschini Sebastian Cremaschini Dipartimento di Fisica e Astronomia “G. Galilei”, Università di Padova, Via Marzolo 8, 35131 Padova, Italy More by Sebastian Cremaschini Orcidhttps://orcid.org/0000-0002-6416-5782 , Alberto Cattelan, Davide Ferraro, Daniele Filippi, Filippo Marinello, Alessio Meggiolaro, Matteo Pierno, Cinzia Sada, Annamaria Zaltron*, Paolo Umari*, and Giampaolo Mistura

Trifurcated Splitting of Water Droplets on Engineered Lithium Niobate Surfaces

Applications: Beam Shaping / Beam Steering,Optical Trapping /-Tweezers