Papers & References

Complex optically computed phase microscopy for label-free sub-cellular imaging

Discontinued Devices LC-R 720 Spatial Light Modulators
Imaging/ Image Processing Microscopy Phase Measurement / Phase Retrieval
Published on:
Authors: Xuan Liu, Yuwei Liu, Shupei Yu, and Yuanwei Zhang
Abstract:

In this study, we investigated a complex optically computed phase microscopy (complex-OCPM) technology. Based on a low coherence interferometer and an innovative optical computation approach, the complex-OCPM imaging system achieves depth resolved quantitative phase measurement. Particularly, the complex-OCPM imaging system directly measures the complex amplitude of the optical field emerging from the sample, extracts the phase as the argument of a complex signal, and achieves a high spatial resolution in phase imaging. We evaluated the performance of complex-OCPM imaging using resolution targets and live cells. Our results show that the complex-OCPM system achieves quantitative phase imaging with sub-cellular resolution on label-free cells.

Open Access

Publication: Optics Continuum
Issue/Year: Opt. Continuum 2, 793-800 (2023)
DOI: 10.1364/OPTCON.486396
Link: https://doi.org/10.1364/OPTCON.486396
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