Papers & References

Rapid, high-contrast, and steady volumetric imaging with Bessel-beam-based two-photon fluorescence microscopy

PLUTO / PLUTO-2 Spatial Light Modulators
Microscopy
Published on:
Authors: Yongqiang Chen, Chenggui Luo, Shiqi Wang, Yanping Li, Binglin Shen, Rui Hu, Junle Qu, Liwei Liu
Abstract:

Two-photon fluorescence microscopy (TPFM) excited by Gaussian beams requires axial tomographic scanning for three-dimensional (3D) volumetric imaging, which is a time-consuming process, and the slow imaging speed hinders its application for in vivo brain imaging. The Bessel focus, characterized by an extended depth of focus and constant resolution, facilitates the projection of a 3D volume onto a two-dimensional image, which significantly enhances the speed of volumetric imaging.

Open Access

Publication: Journal of Biomedical Optics
Issue/Year: Journal of Biomedical Optics, Vol. 29, Issue 1, 016501 (January 2024)
DOI: 10.1117/1.JBO.29.1.016501
Link: https://doi.org/10.1117/1.JBO.29.1.016501
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