Axial resolution enhancement for planar Airy beam light-sheet microscopy via the complementary beam subtraction method

PLUTO / PLUTO-2 Spatial Light Modulators
Higher Order Modes / Optical Vortex / OAM Microscopy
Published on:
Authors: Liu, Chao; Yu, Xianghua; Bai, Chen; Li, Xing; Zhou, Yuan; Yan, Shaohui; Min, Junwei; Dan, Dan; Li, Runze; Gu, Shuangyu & Yao, Baoli
Abstract:
“Airy beam light-sheet illumination can extend the field of view (FOV) of light-sheet fluorescence microscopy due to the unique propagation properties of non-diffraction and self-acceleration. However, the side lobes create undesirable out-of-focus background, leading to poor axial resolution and low image contrast. Here, we propose an Airy complementary beam subtraction (ACBS) method to improve the axial resolution while keeping the extended FOV. By scanning the optimized designed complementary beam that has two main lobes (TML), the generated complementary light-sheet has almost identical intensity distribution to that of the planar Airy light-sheet except for the central lobe. Subtraction of the two images acquired by double exposure respectively using the planar Airy light-sheet and the planar TML light-sheet can effectively suppress the influence of the out-of-focus background. The axial resolution improves from ∼4µm to 1.2 µm. The imaging performance was demonstrated by imaging specimens of aspergillus conidiophores and GFP labeled mouse brain section. The results show that the ACBS method enables the Airy beam light-sheet fluorescence microscopy to obtain better imaging quality.”

Restricted Access

Publication: Applied Optics
Issue/Year: Applied Optics, Volume 60; Number 32; Pages 10239; 2021
DOI: 10.1364/ao.441070
Link: https://doi.org/10.1364/ao.441070

Related Papers

PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Jixiang Wang, Xin Xu, Hong Ye, Xin Zhang, and Guohua Shi

Efficient generation of high-contrast Bessel light sheets: a flexible method for improved imaging

Applications: Beam Shaping / Beam Steering,Bessel-/ Airy Beam Generation,Imaging/ Image Processing,Microscopy
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Treerathat Chomchok, Pemika Hirankittiwong, Apichart Pattanaporkratana, Bussayamas Phettong, Natthawat Hongkanchanakul, Pongthep Prajongtat, Tyler R. Hatch, Dharmendra Pratap Singh, and Nattaporn Chattham

Rotation of liquid crystal microdroplets in the intensity minima of an optical vortex beam

Applications: Higher Order Modes / Optical Vortex / OAM,Optical Trapping /-Tweezers
LETO / LETO-3 Spatial Light Modulators
Authors:Zheng Yuan, Chenchen Zhang, Yuan Gao, Wenxiang Yan, Xian Long, Zhi-Cheng Ren, Xi-Lin Wang, Jianping Ding, and Hui-Tian Wang

Dual-curvilinear beam enabled tunable manipulation of high- and low-refractive-index particles

Applications: Higher Order Modes / Optical Vortex / OAM,Optical Trapping /-Tweezers
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Juntao Hu, Wenjun Wei, Xinzhong Li, and Yixian Qian

Generation of polygonal non-diffracting beams via angular spectral phases

Applications: Beam Shaping / Beam Steering,Higher Order Modes / Optical Vortex / OAM,Microscopy
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:M. P. Morales Rodríguez, E. García Herrera, O. Magaña Loaiza, B. Perez-Garcia, F. Marroquín Gutíerrez, B. M. Rodríguez-Lara

Spatial light mode analogues of generalized quantum coherent states

Applications: Beam Shaping / Beam Steering,Higher Order Modes / Optical Vortex / OAM
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Tong Zhou, Yuchao Hong, Jiantai Dou, Jiaqing Xu, Bo Li, Youyou Hu

Generation of multiple rotationally-symmetric power-exponent-phase vortex beams on a spatial arbitrary distribution by using holographic phase control techniques

Applications: Adaptive Optics / Wavefront Control,Higher Order Modes / Optical Vortex / OAM
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Wangting Zhou, Hui Xie, Kezhou Li, Zhiyuan Sun, Jiangshan He, Zhen Yuan, Xunbin Wei, Xueli Chen

Flexible depth-of-focus, depth-invariant resolution photoacoustic microscopy with Airy beam

Applications: Bessel-/ Airy Beam Generation,Microscopy,Pulse Application / -Shaping
LETO / LETO-3 Spatial Light Modulators
Authors:Manuel Hüpfel and Gerd Ulrich Nienhaus

Beam shaping in light-sheet microscopy: an experimental analysis

Applications: Beam Shaping / Beam Steering,Microscopy
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Bohnishikha Ghosh, Anat Daniel, Bernard Gorzkowski, Aleksandr Y. Bekshaev, Radek Lapkiewicz, and Konstantin Y. Bliokh

Canonical and Poynting currents in propagation and diffraction of structured light: tutorial

Applications: Higher Order Modes / Optical Vortex / OAM
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Dotan Halevi, Boaz Lubotzky, Kfir Sulimany, Eric G. Bowes, Jennifer A. Hollingsworth, Yaron Bromberg, Ronen Rapaport

High-dimensional quantum key distribution using orbital angular momentum of single photons from a colloidal quantum dot at room temperature

Applications: Higher Order Modes / Optical Vortex / OAM,Optical Communication,Optical Computing / Quantum Optics
GAEA / GAEA-2 Spatial Light Modulators
Authors:Wenxiang Yan, Yuan Gao, Zheng Yuan, Xian Long, Zhaozhong Chen, Zhi-Cheng Ren, Xi-Lin Wang, Jianping Ding, and Hui-Tian Wang

Energy-flow-reversing dynamics in vortex beams: OAM-independent propagation and enhanced resilience

Applications: Beam Shaping / Beam Steering,Higher Order Modes / Optical Vortex / OAM
GAEA / GAEA-2 Spatial Light Modulators
Authors:Xiaofei Li, Xin Liu, Quanying Wu, Jun Zeng, Yangjian Cai, Sergey A. Ponomarenko, Chunhao Liang

Prime number factorization with light beams carrying orbital angular momentum

Applications: Deep Learning / Neuronal Network,Digital-/ Computer Holography/ CGH,Higher Order Modes / Optical Vortex / OAM
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Shuo Liu, Yi Zheng, Huiming Xiao, Shengxiang Shan, Xuejuan Liu, Sohail Ahmad, Anwar Manzoor Rana, Shubo Cheng, Wenxing Yang, Shaohua Tao

Generation of a controllable multi-spiral beam by using the modulated helico-conical phases

Applications: Beam Shaping / Beam Steering,Higher Order Modes / Optical Vortex / OAM,Optical Trapping /-Tweezers
GAEA / GAEA-2 PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Fang, Xinyuan / Hu, Xiaonan / Li, Baoli / Su, Hang / Cheng, Ke / Luan, Haitao / Gu, Min

Orbital angular momentum-mediated machine learning for high-accuracy mode-feature encoding

Applications: Deep Learning / Neuronal Network,Higher Order Modes / Optical Vortex / OAM
GAEA / GAEA-2 Spatial Light Modulators
Authors: Zhongzheng Lin, Weihang Zhong, Lixun Wu, Lin He, Hongjia Chen, Jianqi Hu, Yujie Chen, Siyuan Yu

Azimuthal beam shaping in orbital angular momentum basis

Applications: Beam Shaping / Beam Steering,Higher Order Modes / Optical Vortex / OAM
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Nikhil Vangety, P.M. Pooja, Anirban Majee, Sourabh Roy

Learning-enabled recognition of LG beams from multimode fiber specklegrams

Applications: Higher Order Modes / Optical Vortex / OAM
PLUTO / PLUTO-2 Spatial Light Modulators
Authors: Yongqiang Chen, Chenggui Luo, Shiqi Wang, Yanping Li, Binglin Shen, Rui Hu, Junle Qu, Liwei Liu

Rapid, high-contrast, and steady volumetric imaging with Bessel-beam-based two-photon fluorescence microscopy

Applications: Microscopy
LETO / LETO-3 Spatial Light Modulators
Authors:Jian He, Jiahao Chen, Yimin Zhou, Yiqing Xu, Yongzhou Ni, Fei Wang, Yangjian Cai, and Guoquan Zhou

Realization of a circularly transformed Airyprime beam with powerful autofocusing ability

Applications: Bessel-/ Airy Beam Generation,Higher Order Modes / Optical Vortex / OAM
LETO / LETO-3 Spatial Light Modulators
Authors: Siqing Dai, Andrei Kobitski, Amirhossein Barati Sedeh, Süheyla Eroğlu-Kayıkçı, Lennart Hilbert, and G. Ulrich Nienhaus

Photon-Efficient Aberration Correction for 3D-STED Imaging of Thick Biological Specimens Using Sensorless Adaptive Optics

Applications: Adaptive Optics / Wavefront Control,Beam Shaping / Beam Steering,Microscopy
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Olivier Emile and Janine Emile

Optical orbital angular momentum transfer to electronic currents

Applications: Higher Order Modes / Optical Vortex / OAM
GAEA / GAEA-2 Spatial Light Modulators
Authors:Kaibo Yang, Hao Luo, Yidan Zhang, Peng Li, Feng Wen, Yuzong Gu, Zhenkun Wu

Modulating and identifying an arbitrary curvilinear phased optical vortex array of high-order orbital angular momentum

Applications: Complex Modulation,Higher Order Modes / Optical Vortex / OAM,Optical Communication
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Jawahar Prabhakar Desai, Joseph Rosen

Resolution-enhanced imaging by interferenceless coded aperture correlation holography with two competing methods of phase mask synthesis

Applications: Microscopy
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Tianhong Wang, Viet Tran, Pascal Bassène, Edwin Fohtung, Trevor Rhone, and Moussa N’Gom

Adaptive methods of generating complex light arrays

Applications: Bessel-/ Airy Beam Generation,Complex Modulation,Higher Order Modes / Optical Vortex / OAM
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Krishnendu Samanta, Anupriya Tiwari, Samsheerali P.T., Joby Joseph

Improving resolution in two orthogonal orientations from a single-shot digital holographic microscopy

Applications: Microscopy