Phase contrast enhancement in microscopy using spiral phase filtering
Authors: Guohai Situ, Michael Warber, Giancarlo Pedrini, Wolfgang Osten.
“We demonstrate two methods based on Fourier plane filtering using (a) a fractional spiral phase plate (SPP) and (b) an off-axial SPP for phase contrast enhancement in optical microscopy. In comparison to previous works, a spatially incoherent LED is used in the Köhler illumination as the light source to illuminate the biological specimen. We demonstrate that both these methods can transform the phase specimen into a relief-like view even under such illumination. The degree and orientation of enhancement can be controlled by changing the phase structure of the filter. The SPP is displayed on a phase-only spatial light modulator, and can be integrated into the optical path of standard microscopes.”
Publication: Optics Communications
Issue/Year: Optics Communications, Volume 283, Issue 7, 1 April 2010, Pages 1273–1277