Papers & References

Multiplexed force measurements on live cells with holographic optical tweezers

PLUTO / PLUTO-2 Spatial Light Modulators
Microscopy
Published on:
Authors: Cecile O. Mejean, Andrew W. Schaefer, Eleanor A. Millman, Paul Forscher, and Eric R. Dufresne
Abstract:
“We describe open-loop and closed-loop multiplexed force measurements using holographic optical tweezers. We quantify the performance of our novel video-based control system in a driven suspension of colloidal particles. We demonstrate our system’s abilities with the measurement of the mechanical coupling between Aplysia bag cell growth cones and beads functionalized with the neuronal cell adhesion molecule, apCAM. We show that cells form linkages which couple beads to the underlying cytoskeleton. These linkages are intermittent, stochastic and heterogeneous across beads distributed near the leading edge of a single growth cone.”

Open Access

Publication: Optics Express
Issue/Year: Optics Express, Vol. 17, Issue 8, pp. 6209-6217 (2009)
DOI: 10.1364/OE.17.006209
Link: https://doi.org/10.1364/OE.17.006209
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