Coherent optical adaptive technique improves the spatial resolution of STED microscopy in thick samples

PLUTO / PLUTO-2 Spatial Light Modulators
Microscopy
Published on:
Authors: Wei Yan and Yanlong Yang and Yu Tan and Xun Chen and Yang Li and Junle Qu and Tong Ye
Abstract:

“Stimulated emission depletion (STED) microscopy is one of far-field optical microscopy techniques that can provide sub-diffraction spatial resolution. The spatial resolution of the STED microscopy is determined by the specially engineered beam profile of the depletion beam and its power. However, the beam profile of the depletion beam may be distorted due to aberrations of optical systems and inhomogeneity of a specimen’s optical properties, resulting in a compromised spatial resolution. The situation gets deteriorated when thick samples are imaged. In the worst case, the severe distortion of the depletion beam profile may cause complete loss of the super-resolution effect no matter how much depletion power is applied to specimens. Previously several adaptive optics approaches have been explored to compensate aberrations of systems and specimens. However, it is difficult to correct the complicated high-order optical aberrations of specimens. In this report, we demonstrate that the complicated distorted wavefront from a thick phantom sample can be measured by using the coherent optical adaptive technique. The full correction can effectively maintain and improve spatial resolution in imaging thick samples.”

Open Access

Publication: Photonics Research
Issue/Year: Photonics Research, Vol. 5, Issue3, pp. 176-181 (2017)
DOI: 10.1364/PRJ.5.000176
Link: https://doi.org/10.1364/PRJ.5.000176

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