Endogenous SHG and 2PEF coherence imaging of substructures in neurons in 3D

PLUTO / PLUTO-2 Spatial Light Modulators
Imaging/ Image Processing Microscopy
Published on:
Authors: Carlos Macias-Romero, Claire Teulon, Marie Didier, and Sylvie Roke
Abstract:

“Neuronal morphology, long-distance transport and signalling critically depend on the organization of microtubules in the cytoskeleton. Second harmonic generation (SHG) imaging has been recognized as a potentially powerful tool for in situ label-free neuroimaging with specific sensitivity to microtubules. We study here the structural organization of microtubules in living neurons using a wide-field multiphoton microscope that performs 3D imaging using a structured illumination. This microscope allows label-free high throughput imaging of living mammalian neurons. We show that we can image structural correlations by taking advantage of the structured illumination and the coherence of the emitted light. The result allows us to study the microtubule organization throughout the development of the neuron and to differentiate between the regions of the cytoskeleton in the matured neuron.”

Open Access

Publication: Optics Express
Issue/Year: Vol. 27, Issue 3, pp. 2235-2247 (2019)
DOI: 10.1364/OE.27.002235
Link: https://doi.org/10.1364/OE.27.002235

Related Papers

LC 2012 Spatial Light Modulators
Authors:Weng, Xiaojing / Feng, Ji / Perry, Altai / Vuong, Luat T.

Non-Line-of-Sight Full-Stokes Polarimetric Imaging with Solution-Processed Metagratings and Shallow Neural Networks

Applications: Deep Learning / Neuronal Network,Imaging/ Image Processing,Polarization Generation
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Yun, Xue / Liang, Yansheng / He, Minru / Zhao, Tianyu / Wang, Shaowei / Lei, Ming / Lei, Ming

Optical trapping of gold microparticles using linearly polarized, centrally obstructed Gaussian beams

Applications: Microscopy,Optical Trapping /-Tweezers
GAEA Spatial Light Modulators
Authors:Tilmon, Brevin; Sun, Zhanghao; Koppal, Sanjeev; Wu, Yicheng; Evangelidis, Georgios; Zahreddine, Ramzi; Krishnan, Guru; Ma, Sizhuo & Wang, Jian

Energy-Efficient Adaptive 3D Sensing

Applications: Digital-/ Computer Holography/ CGH,Imaging/ Image Processing,Misc.: Speckle / Characterization / Metrology
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Zhou, Chaohui; Xin, Jun; Li, Yanan; Lu, Xiao-Ming

Measuring small displacements of an optical point source with digital holography

Applications: Digital-/ Computer Holography/ CGH,Imaging/ Image Processing
LC 2012 Spatial Light Modulators
Authors:Tsukada, Takumi / Watanabe, Wataru

Central wavelength estimation in spectral imaging behind a diffuser via deep learning

Applications: Deep Learning / Neuronal Network,Imaging/ Image Processing,Misc.: Speckle / Characterization / Metrology
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Han, Tongyu; Peng, Tong; Li, Runze; Wang, Kaige; Sun, Dan & Yao, Baoli

Extending the Imaging Depth of Field through Scattering Media by Wavefront Shaping of Non-Diffraction Beams

Applications: Bessel-/ Airy Beam Generation,Imaging/ Image Processing,Turbid-/ Opaque Media /Multi Scattering
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Zhou, Dongxu; Zhang, Lu; Zhang, Hongzhi; Zhang, Guoquan

Ghost images with controllable visibility and spatial resolution

Applications: Imaging/ Image Processing,Misc.: Speckle / Characterization / Metrology
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Ye, Zhiyuan; Hou, Wanting; Zhao, Jilun; Wang, Hai-Bo & Xiong, Jun

Computational holographic ghost diffraction

Applications: Imaging/ Image Processing,Phase Measurement / Phase Retrieval
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Yudaev, Andrey; Kiselev, Alexander; Shashkova, Inna; Tavrov, Alexander; Lipatov, Alexander & Korablev, Oleg

Wavefront Sensing by a Common-Path Interferometer for Wavefront Correction in Phase and Amplitude by a Liquid Crystal Spatial Light Modulator Aiming the Exoplanet Direct Imaging

Applications: Adaptive Optics / Wavefront Control,Imaging/ Image Processing,Phase Measurement / Phase Retrieval
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Xu, Ning; Bohndiek, Sarah E.; Li, Zexing; Zhang, Cilong & Tan, Qiaofeng

Mechanical-scan-free and multi-color super-resolutionimaging with diffractive spot array illumination

Applications: Microscopy
LC 2012 Spatial Light Modulators
Authors:Neto, Jose Reinaldo da Cunha Santos Aroso Vieira da Silva; Nakamura, Tomoya; Makihara, Yasushi & Yagi, Yasushi

Extended Depth-of-Field Lensless Imaging using an Optimized Radial Mask

Applications: Imaging/ Image Processing
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Luo, Tianpeng; Yuan, Jing; Chang, Jin; Dai, Yanfeng; Gong, Hui; Luo, Qingming & Yang, Xiaoquan

Resolution and uniformity improvement of parallel confocal microscopy based on microlens arrays and a spatial light modulator

Applications: Microscopy
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Navarro, Gilberto

Modulation of Non-diffracting Hermite Gaussian Beams and Nonlinear Optical Microscopy for Nanoscale Sulfur Imaging

Applications: Beam Shaping / Beam Steering,Higher Order Modes / Optical Vortex / OAM,Microscopy
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Guo, Yong; Wang, Luwei; Zhu, Yinru; Gao, Xinwei; Weng, Xiaoyu; Liu, Jinyuan; Yan, Wei & Qu, Junle

Improving longitudinal resolution of Airy beams two-photon volume imaging with fluorescence lifetime imaging

Applications: Bessel-/ Airy Beam Generation,Microscopy
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Madan, Ivan; Leccese, Veronica; Mazur, Adam; Barantani, Francesco; LaGrange, Thomas; Sapozhnik, Alexey; Tengdin, Phoebe M.; Gargiulo, Simone; Rotunno, Enzo; Olaya, Jean-Christophe; Kaminer, Ido; Grillo, Vincenzo; de Abajo, F. Javier Garcia; Carbone, Fabrizio & Vanacore, Giovanni Maria

Ultrafast Transverse Modulation of Free Electrons by Interaction with Shaped Optical Fields

Applications: Beam Shaping / Beam Steering,Higher Order Modes / Optical Vortex / OAM,Microscopy
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Rowe, Connor M. L.; Cortes, Luis Romero; de Chatellus, Hugues Guillet; Seghilani, Mohamed & Azana, Jose

Generalized Talbot Self-Healing of Periodic Images

Applications: Imaging/ Image Processing
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Reda, Francesco; Salvatore, Marcella; Borbone, Fabio; Maddalena, Pasqualino; Ambrosio, Antonio & Oscurato, Stefano Luigi

Varifocal diffractive lenses for multi-depth microscope imaging

Applications: AR/VR/MR / Holographic Display,Imaging/ Image Processing,Lithography
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Dou, Jiazhen; Dong, Chen; Dai, Siqing; Mi, Jingyu; Luo, Xiangyuan; Di, Jianglei; Zhang, Jiwei & Zhao, Jianlin

High-resolution surface plasmon resonance holographic microscopy based on symmetrical excitation

Applications: Digital-/ Computer Holography/ CGH,Microscopy
GAEA Spatial Light Modulators
Authors:Wu, Peng; Zhang, Dejie; Yuan, Jing; Zeng, Shaoqun; Gong, Hui; Luo, Qingming & Yang, Xiaoquan

Large depth-of-field fluorescence microscopy based on deep learning supported by Fresnel incoherent correlation holography

Applications: Digital-/ Computer Holography/ CGH,Microscopy,Misc.: Speckle / Characterization / Metrology
Spatial Light Modulators
Authors:Cheremkhin, Pavel; Evtikhiev, Nikolay; Krasnov, Vitaly; Ryabcev, Ilya; Shifrina, Anna & Starikov, Rostislav

Lensless Optical Encryption of Multilevel Digital Data Containers Using Spatially Incoherent Illumination

Applications: Digital-/ Computer Holography/ CGH,Imaging/ Image Processing,Optical Communication
LC-R 720 Spatial Light Modulators
Authors:Liu, Xuan; Wan, Zhaoxiong; Zhang, Yuanwei & Liu, Yuwei

Optically computed phase microscopy for quantitative dynamic imaging of label-free cells and nanoparticles

Applications: Imaging/ Image Processing,Microscopy
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Bueno, J.M.; Hernández, G.; Skorsetz, M.; Artal, P.

Improving Multiphoton Microscopy by Combining Spherical Aberration Patterns and Variable Axicons

Applications: Adaptive Optics / Wavefront Control,Microscopy
LC 2012 Spatial Light Modulators
Authors:Lai, Xuetian; Li, Qiongyao; Chen, Ziyang; Shao, Xiaopeng & Pu, Jixiong

Reconstructing images of two adjacent objects passing through scattering medium via deep learning

Applications: Imaging/ Image Processing,Turbid-/ Opaque Media /Multi Scattering
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Bulbul, Angika; Hai, Nathaniel & Rosen, Joseph

Coded aperture correlation holography (COACH) with a superior lateral resolution of FINCH and axial resolution of conventional direct imaging systems

Applications: Digital-/ Computer Holography/ CGH,Imaging/ Image Processing,Misc.: Speckle / Characterization / Metrology