Structured illumination in total internal reflection fluorescence microscopy using a spatial light modulator

PLUTO / PLUTO-2 Spatial Light Modulators
AR/VR/MR / Holographic Display Imaging/ Image Processing Microscopy Misc.: Speckle / Characterization / Metrology
Published on:
Authors: Reto Fiolka, Markus Beck, and Andreas Stemmer
Abstract:

“In wide-field fluorescence microscopy, illuminating the specimen with evanescent standing waves increases lateral resolution more than twofold. We report a versatile setup for standing-wave illumination in total internal reflection fluorescence microscopy. An adjustable diffraction grating written on a phase-only spatial light modulator controls the illumination field. Selecting appropriate diffraction orders and displaying a sheared (tilted) diffraction grating allows one to tune the penetration depth in very fine steps. The setup achieves 91 nm lateral resolution for green emission.”

Restricted Access

Publication: Optics Letters
Issue/Year: Optics Letters, Vol. 33, Issue 14, pp. 1629-1631
DOI: 10.1364/OL.33.001629
Link: https://doi.org/10.1364/OL.33.001629

Related Papers

PLUTO / PLUTO-2 Spatial Light Modulators
Authors: Yongqiang Chen, Chenggui Luo, Shiqi Wang, Yanping Li, Binglin Shen, Rui Hu, Junle Qu, Liwei Liu

Rapid, high-contrast, and steady volumetric imaging with Bessel-beam-based two-photon fluorescence microscopy

Applications: Microscopy
LETO / LETO-3 Spatial Light Modulators
Authors: Siqing Dai, Andrei Kobitski, Amirhossein Barati Sedeh, Süheyla Eroğlu-Kayıkçı, Lennart Hilbert, and G. Ulrich Nienhaus

Photon-Efficient Aberration Correction for 3D-STED Imaging of Thick Biological Specimens Using Sensorless Adaptive Optics

Applications: Adaptive Optics / Wavefront Control,Beam Shaping / Beam Steering,Microscopy
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Jiuru He, Pengwei Zhang, Jianpo Su, Junqiao Wang, Yongzhi Tian, Yongsheng Hu, and Fengying Ma

Edge enhancement in three-dimensional vortex imaging based on FINCH by Bessel-like spiral phase modulation

Applications: Imaging/ Image Processing
LETO / LETO-3 Spatial Light Modulators
Authors:Wenxue Zhang, Yuhong Wan, Tianlong Man, Hongqiang Zhou

Computational incoherent edge enhancement imaging based on self-interference digital holography

Applications: Imaging/ Image Processing
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Jawahar Prabhakar Desai, Joseph Rosen

Resolution-enhanced imaging by interferenceless coded aperture correlation holography with two competing methods of phase mask synthesis

Applications: Microscopy
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Dejin Zhang, Wei Sheng, Yaoyao Shi, and Youwen Liu

Imaging objects hidden inside the strongly scattering media based on bidirectional ghost imaging

Applications: Imaging/ Image Processing,Turbid-/ Opaque Media /Multi Scattering
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Krishnendu Samanta, Anupriya Tiwari, Samsheerali P.T., Joby Joseph

Improving resolution in two orthogonal orientations from a single-shot digital holographic microscopy

Applications: Microscopy
PLUTO / PLUTO-2 Spatial Light Modulators
Authors: Koray Kavaklı , Liang Shi , Hakan Urey , Wojciech Matusik , Kaan Akşit

Multi-color Holograms Improve Brightness in Holographic Displays

Applications: AR/VR/MR / Holographic Display,Digital-/ Computer Holography/ CGH
PLUTO / PLUTO-2 Spatial Light Modulators
Authors: Eric Markley , Nathan Matsuda , Florian Schiffers , Oliver Cossairt , Grace Kuo

Simultaneous Color Computer Generated Holography

Applications: AR/VR/MR / Holographic Display,Digital-/ Computer Holography/ CGH
PLUTO / PLUTO-2 Spatial Light Modulators
Authors: Grace Kuo , Florian Schiffers , Douglas Lanman , Oliver Cossairt , Nathan Matsuda

Multisource Holography

Applications: AR/VR/MR / Holographic Display,Deep Learning / Neuronal Network,Digital-/ Computer Holography/ CGH
LETO / LETO-3 Spatial Light Modulators
Authors: Seung-Woo Nam , Youngjin Kim , Dongyeon Kim , Yoonchan Jeong

Depolarized Holography with Polarization-Multiplexing Metasurface

Applications: AR/VR/MR / Holographic Display,Digital-/ Computer Holography/ CGH,Materials Processing / Optical Fabrication
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Zheyu Wu, Ran Gao, Sitong Zhou, Fei Wang, Zhipei Li, Huan Chang, Dong Guo, Xiangjun Xin, Qi Zhang, Feng Tian, Qiang Wu

Robust Super-Resolution Image Transmission Based on a Ring Core Fiber with Orbital Angular Momentum

Applications: Deep Learning / Neuronal Network,Higher Order Modes / Optical Vortex / OAM,Imaging/ Image Processing
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Chané Moodley and Andrew Forbes

All-digital quantum ghost imaging: tutorial

Applications: Imaging/ Image Processing
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Emory L. Jenkins and Derek J. Burrell

Improved active-tracking performance through Hadamard speckle contrast reduction

Applications: Misc.: Speckle / Characterization / Metrology
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Qingwei Liu, Jing Chen, Bingsen Qiu, Yongtian Wang, and Juan Liu

DCPNet: a dual-channel parallel deep neural network for high quality computer-generated holography

Applications: AR/VR/MR / Holographic Display,Complex Modulation,Deep Learning / Neuronal Network,Digital-/ Computer Holography/ CGH
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Kumar, Rahul, Naveen K. Nishchal, and Ayman Alfalou

Improving the False Alarm Capability of the Extended Maximum Average Correlation Height Filter

Applications: Imaging/ Image Processing
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Rafael Barros, Subhajit Bej, Markus Hiekkamäki, Marco Ornigotti, Robert Fickler

High-order aberrations of vortex constellations

Applications: Higher Order Modes / Optical Vortex / OAM,Misc.: Speckle / Characterization / Metrology
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Jia-Qi Lü, Tian-Yu Cheng, Ji-Xiang Guo, Jin-Song Li, Yulei Wang, Zhiwei Lu

Robust measurement of angular velocity based on rotational Doppler effect in misaligned illumination

Applications: Beam Shaping / Beam Steering,Higher Order Modes / Optical Vortex / OAM,Misc.: Speckle / Characterization / Metrology
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Xinxing Xia, Weisen Wang, Frank Guan, Furong Yang, Xinghua Shui, Huadong Zheng, Yingjie Yu, and Yifan Peng

Exploring angular-steering illumination-based eyebox expansion for holographic displays

Applications: AR/VR/MR / Holographic Display,Digital-/ Computer Holography/ CGH
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Zhang, Yantong, Huiling Huang, Feibin Wu, Jun Han, Yi Yang, and Ruyi Li

Imaging through a Scattering Medium under Different Intensities of Ambient Light Interference

Applications: Deep Learning / Neuronal Network,Imaging/ Image Processing,Turbid-/ Opaque Media /Multi Scattering
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Huadong Zheng, Zhen Wang, Junchang Peng, Xinxing Xia, Yingjie Yu

Multiplane full-color phase-only holograms generation based on iterative algorithm with a modified amplitude constraint

Applications: AR/VR/MR / Holographic Display,Digital-/ Computer Holography/ CGH
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:León Schweickhardt , Dirk Stöbener und Andreas Fischer

Specklebasierte Charakterisierung anisotroper Oberflächen

Applications: Imaging/ Image Processing
GAEA Spatial Light Modulators
Authors:Andreas Georgiou, Jia Jia, Charlie Hewitt, Changwon Jang, Jacob Hadnett-Hunter, Jinsoo Jeong, Joel Kollin

A holographic near-eye display with glass form factor and spectacle-free operation

Applications: AR/VR/MR / Holographic Display,Digital-/ Computer Holography/ CGH
GAEA Spatial Light Modulators
Authors:Yingsi Qin, Wei-Yu Chen, Matthew O’Toole, Aswin C. Sankaranarayanan

Split-Lohmann Multifocal Displays

Applications: AR/VR/MR / Holographic Display,Beam Shaping / Beam Steering,Digital-/ Computer Holography/ CGH