Structured illumination in total internal reflection fluorescence microscopy using a spatial light modulator

PLUTO / PLUTO-2 Spatial Light Modulators
AR/VR/MR / Holographic Display Imaging/ Image Processing Microscopy Misc.: Speckle / Characterization / Metrology
Published on:
Authors: Reto Fiolka, Markus Beck, and Andreas Stemmer
Abstract:

“In wide-field fluorescence microscopy, illuminating the specimen with evanescent standing waves increases lateral resolution more than twofold. We report a versatile setup for standing-wave illumination in total internal reflection fluorescence microscopy. An adjustable diffraction grating written on a phase-only spatial light modulator controls the illumination field. Selecting appropriate diffraction orders and displaying a sheared (tilted) diffraction grating allows one to tune the penetration depth in very fine steps. The setup achieves 91 nm lateral resolution for green emission.”

Restricted Access

Publication: Optics Letters
Issue/Year: Optics Letters, Vol. 33, Issue 14, pp. 1629-1631
DOI: 10.1364/OL.33.001629
Link: https://doi.org/10.1364/OL.33.001629

Related Papers

PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Kenneth Chen, Anzhou Wen, Yunxiang Zhang, Praneeth Chakravarthula and Qi Sun

View synthesis for 3D computer-generated holograms using deep neural fields

Applications: AR/VR/MR / Holographic Display,Deep Learning / Neuronal Network,Digital-/ Computer Holography/ CGH
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Adolphe Ndagijimana, Iñigo Ederra and Miguel Heredia Conde

Single-Pixel Compressive Terahertz 3D Imaging

Applications: Complex Modulation,Imaging/ Image Processing
LETO / LETO-3 Spatial Light Modulators
Authors:Augusto Arias, Susanna P. Clement, Pablo Artal and Siegfried Wahl

Chromatic imaging properties of myopia control spectacle lenses

Applications: Adaptive Optics / Wavefront Control,Imaging/ Image Processing
GAEA / GAEA-2 Spatial Light Modulators
Authors:Zhenqi Xu, Junmin Leng, Ping Dai and Chao Wang

DSCCNet for high-quality 4K computer-generated holograms

Applications: AR/VR/MR / Holographic Display,Complex Modulation,Deep Learning / Neuronal Network,Digital-/ Computer Holography/ CGH
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Yu-Chen Chen, Shi-Xuan Mi, Ya-Ping Tian, Xiao-Bo Hu, Qi-Yao Yuan, Khian-Hooi Chew and Rui-Pin Chen

Adaptive Vectorial Restoration from Dynamic Speckle Patterns Through Biological Scattering Media Based on Deep Learning

Applications: Adaptive Optics / Wavefront Control,Deep Learning / Neuronal Network,Digital-/ Computer Holography/ CGH,Imaging/ Image Processing,Microscopy,Misc.: Speckle / Characterization / Metrology,Phase Measurement / Phase Retrieval,Polarization Generation,Turbid-/ Opaque Media /Multi Scattering
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Wenjun Yi, Shuyue Zhu, Meicheng Fu, Nan Gu, Junli Qi, Siyu Liu, Mengjun Zhu, Ping Wang, Xin Chen, Yi Zhang, Hongyu Zhang, Yao Xu, Junyi Du, Peng Xiong, Zhaohua Dong, Luobing Dong, Qiong Liu and Xiujian Li

Relay-projection microscopic telescopy

Applications: Imaging/ Image Processing,Microscopy
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Allarakha Shikder, Naveen K. Nishchal

Image encryption using arbitrary modes of Hermite-Gaussian beams

Applications: Higher Order Modes / Optical Vortex / OAM,Imaging/ Image Processing,Optical Computing / Quantum Optics
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Qiukun Liao, Shijie Zhang, Yongtian Wang and Juan Liu

High-quality real-time 3D holographic display for real-world scenes based on the optimized layered angular spectrum method

Applications: AR/VR/MR / Holographic Display,Deep Learning / Neuronal Network,Digital-/ Computer Holography/ CGH,Misc.: Speckle / Characterization / Metrology,Phase Measurement / Phase Retrieval
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Jawahar Prabhakar Desai, Vijayakumar Anand and Joseph Rosen

Multiple Incoherent Deconvolutions for Improving the Image Resolution of Diffraction-Limited Imaging Systems

Applications: Imaging/ Image Processing
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Jiajia Wu, Xinkuo Li, Ke Sun, Kai Gao, Chenduan Chen, Jianrong Qiu and Dezhi Tan

Tight Focusing Holographic Network Enables 3D Real Time and Accurate Light Field Modulation

Applications: AR/VR/MR / Holographic Display,Deep Learning / Neuronal Network,Digital-/ Computer Holography/ CGH,Materials Processing / Optical Fabrication
GAEA / GAEA-2 Spatial Light Modulators
Authors:Zhenxing Dong, Yuye Ling, Yan Li and Yikai Su

Motion Hologram: Jointly optimized hologram generation and motion planning for photorealistic 3D displays via reinforcement learning

Applications: AR/VR/MR / Holographic Display,Digital-/ Computer Holography/ CGH
GAEA / GAEA-2 Spatial Light Modulators
Authors:Riku Watanabe, Seok Kang and Yuji Sakamoto

Binocular holographic display with a wide viewing zone using eye-tracking and multiple reconstruction lights

Applications: AR/VR/MR / Holographic Display,Digital-/ Computer Holography/ CGH
LC-R 720 Spatial Light Modulators
Authors:Xuan Liu, Rupak Bhakta, Emily Kryvorutsky and Yuanwei Zhang

Imaging cells and nanoparticles using modulated optically computed phase microscopy

Applications: Imaging/ Image Processing
LC 2012 PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Youjia Gu and Yasuhiro Takaki

See-through characteristics of a holographic contact lens display and design consideration for a spatial light modulator

Applications: AR/VR/MR / Holographic Display
GAEA / GAEA-2 Spatial Light Modulators
Authors:Kanno Tomoki, Ichihashi Yasuyuki, Kakue Takashi, Wakunami Koki, Oi Ryutaro, Shimobaba Tomoyoshi and Ito Tomoyoshi

Projection-Type Holographic Display With Observation Area Expanded Using Time-Division Reproduced Light Generation System

Applications: AR/VR/MR / Holographic Display
LUNA Spatial Light Modulators
Authors:Natsu Kikuchi, Seok Kang and Yuji Sakamoto

Head-mounted projector with a wide visual-field and three degrees of freedom in electro-holography

Applications: AR/VR/MR / Holographic Display
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Zhenting Yan, Huiling Huang, Yili Chen, Hanfei Lin, Ebraheem Farea, Xingzhao Hua and Jun Han

Scattering imaging of moving targets based on recurrent neural networks: a fusion of temporal and phase information

Applications: Deep Learning / Neuronal Network,Imaging/ Image Processing,Turbid-/ Opaque Media /Multi Scattering
LC 2002 Spatial Light Modulators
Authors:Jyoti Bikash Mohapatra, Naveen K. Nishchal and Jyothish Monikantan

Object Recognition in Foggy and Hazy Conditions Using Dark Channel Prior-Based Fringe-Adjusted Joint Transform Correlator

Applications: Digital-/ Computer Holography/ CGH,Imaging/ Image Processing
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Qiwei Fang, Huadong Zheng, Xinxing Xia, Junchang Peng, Tengfei Zhang, Xingyu Lin and Yingjie Yu

Diffraction model-driven neural network with semi-supervised training strategy for real-world 3D holographic photography

Applications: AR/VR/MR / Holographic Display,Deep Learning / Neuronal Network,Digital-/ Computer Holography/ CGH
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Sara Aissati, Tianlun Zou, Sabyasachi Goswami, Len Zheleznyak, Susana Marcos

Visual Quality and Accommodation With Novel Optical Designs for Myopia Control

Applications: Imaging/ Image Processing,Misc.: Speckle / Characterization / Metrology
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Zicheng Huang, Mengyang Shi, Jiahui Ma, Zhishun Guo, Yesheng Gao and Xingzhao Liu

Speckle-free self-supervised learning for scalable imaging through scattering media with unseen condition changes

Applications: Deep Learning / Neuronal Network,Misc.: Speckle / Characterization / Metrology,Turbid-/ Opaque Media /Multi Scattering
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:R. Gottschalk, A. Lindinger

Interference patterns of two-photon excited fluorescence by spatial beam shaping

Applications: Adaptive Optics / Wavefront Control,Imaging/ Image Processing,Microscopy
PLUTO / PLUTO-2 Spatial Light Modulators
Authors:Jacob Kher-Aldeen, Kobi Cohen, Stav Lotan, Kobi Frischwasser, Bergin Gjonaj, Shai Tsesses & Guy Bartal

Dynamic control and manipulation of near-fields using direct feedback

Applications: Beam Shaping / Beam Steering,Microscopy
LETO / LETO-3 Spatial Light Modulators
Authors:Xian Ding, Chenliang Chang, Bo Dai, Qi Wang, Dawei Zhang and Songlin Zhuang

Real-time holographic 3D display using Split–Lohmann Fresnel computer-generated hologram (SL-FCGH)

Applications: AR/VR/MR / Holographic Display,Digital-/ Computer Holography/ CGH