Holographic optical tweezers with real-time hologram calculation using a phase-only modulating LCOS-based SLM at 1064 nm

PLUTO / PLUTO-2 Spatial Light Modulators
Microscopy Optical Trapping /-Tweezers
Published on:
Authors: Andreas Hermerschmidt, Sven Krüger, Tobias Haist, Susanne Zwick, Michael Warber, Wolfgang Osten
Abstract:

“We present a method that enables the generation of arbitrary positioned dual-beam traps without additional hardware in a single-beam holographic optical tweezers setup. By this approach stable trapping at low numerical aperture and long working distance is realized with an inverse standard research microscope. Simulations and first experimental results are presented. Additionally we present first steps towards using the method to realize a holographic 4pi-microscope. We will also give a detailed analysis of the phase-modulating properties and especially the spatial-frequency dependent diffraction efficiency of holograms reconstructed with the phase-only LCOS spatial light modulator used in our system. Finally, accelerated hologram optimization based on the iterative Fourier transform algorithm is done using the graphics processing unit of a consumer graphics board.”

Restricted Access

Publication: SPIE Digital Library
Issue/Year: SPIE Proceedings, Vol. 6905, 690508 (2008),
DOI: 10.1117/12.764649
Link: https://doi.org/10.1117/12.764649

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