Comparative study on resolution enhancements in fluorescence-structured illumination Fresnel incoherent correlation holography

PLUTO / PLUTO-2 Spatial Light Modulators
Adaptive Optics / Wavefront Control Digital-/ Computer Holography/ CGH Microscopy
Published on:
Authors: Jeon, Philjun; Kim, Jongwu; Lee, Heejung; Kwon, Hyuk-Sang & young Kim, Dug
Abstract:
“Fresnel incoherent correlation holography (FINCH) is a new approach for incoherent holography, which also has enhancement in the transverse resolution. Structured illumination microscopy (SIM) is another promising super-resolution technique. SI-FINCH, the combination of SIM and FINCH, has been demonstrated lately for scattering objects. In this study, we extended the application of SI-FINCH toward fluorescent microscopy. We have built a versatile multimodal microscopy system that can obtain images of four different imaging schemes: conventional fluorescence microscopy, FINCH, SIM, and SI-FINCH. Resolution enhancements were demonstrated by comparing the point spread functions (PSFs) of the four different imaging systems by using fluorescence beads of 1-μm diameter.”

Open Access

Publication: Optics Express
Issue/Year: Optics Express, Volume 29; Number 6; Pages 9231; 2021
DOI: 10.1364/oe.417206
Link: https://doi.org/10.1364/oe.417206

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