Fluorescence incoherent color holography

PLUTO / PLUTO-2 Spatial Light Modulators
Digital-/ Computer Holography/ CGH
Published on:
Authors: Joseph Rosen and Gary Brooker
Abstract:

“We present a new imaging method to record multicolor digital holograms from objects emitting fluorescent light. The fluorescent light specific to the emission wavelength of various fluorescent dyes after excitation of three dimensional (3D) objects is recorded on a digital monochrome camera after reflection from a diffractive optical element (DOE). For each wavelength of fluorescent emission, the camera sequentially records three holograms reflected from the DOE, each with a different phase factor of the DOE’s function. The three holograms are superposed in a computer to create a complex valued Fresnel hologram of each fluorescent emission. The holograms for each fluorescent color are further combined in a computer to produce a multicolored fluorescence hologram and 3D color image.”

Open Access

Publication: Optics Express
Issue/Year: Optics Express, Vol. 15, Issue 5, pp. 2244-2250
DOI: 10.1364/OE.15.002244
Link: https://doi.org/10.1364/OE.15.002244

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