Abstract: “Significance: Digital holographic microscopy is widely used to get the quantitative phase information of transparent cells._x000D_
_x000D_
Aim: However, the sample phase is superimposed with aberrations. To quantify the phase information, aberrations need to be fully compensated._x000D_
_x000D_
Approach: We propose a technique to obtain aberration-free phase imaging, using the derivative-based principal component analysis (dPCA)._x000D_
_x000D_
Results: With dPCA, almost all aberrations can be extracted and compensated without requirements on background segmentation, making it efficient and convenient._x000D_
_x000D_
Conclusions: It solves the problem that the conventional principal component analysis (PCA) algorithm cannot compensate the common but intricate higher order cross-term aberrations, such as astigmatism and coma. Moreover, the dPCA strategy proposed here is not only suitable for aberration compensation but also applicable for other cases where there exist cross-terms that cannot be analyzed with the PCA algorithm.”
Open Access
Publication: Journal of Biomedical Optics
Issue/Year: Journal of Biomedical Optics, Volume 26; Number 04; 2021
Authors:Michael Flachhuber, Johannes Scheuchenpflug, Thomas Hilbert, Norbert Danz, Peter Schreiber, Leo M. Wilhelm, Markus Metz, Jean-Christope Olaya, Tobias Reusch
MaMeK: a wide-angle dynamic holographic projection system for human-vehicle communication