Aberration-free digital holographic phase imaging using the derivative-based principal component analysis

Abstract:
“Significance: Digital holographic microscopy is widely used to get the quantitative phase information of transparent cells._x000D_ _x000D_ Aim: However, the sample phase is superimposed with aberrations. To quantify the phase information, aberrations need to be fully compensated._x000D_ _x000D_ Approach: We propose a technique to obtain aberration-free phase imaging, using the derivative-based principal component analysis (dPCA)._x000D_ _x000D_ Results: With dPCA, almost all aberrations can be extracted and compensated without requirements on background segmentation, making it efficient and convenient._x000D_ _x000D_ Conclusions: It solves the problem that the conventional principal component analysis (PCA) algorithm cannot compensate the common but intricate higher order cross-term aberrations, such as astigmatism and coma. Moreover, the dPCA strategy proposed here is not only suitable for aberration compensation but also applicable for other cases where there exist cross-terms that cannot be analyzed with the PCA algorithm.”