Full 3D translational and rotational optical control of multiple rod-shaped bacteria

Author(s): Florian Hörner, Mike Woerdemann, Stephanie Müller, Berenike Maier, Cornelia Denz Abstract: “The class of rod-shaped bacteria is an important example of non-spherical objects where defined alignment is desired for the observation of intracellular processes or studies of the flagella. However, all available methods for orientational control of rod-shaped bacteria are either limited with respect to the accessible rotational axes or feasible angles or restricted to one single bacterium. In this paper we demonstrate a scheme to orientate rod-shaped bacteria with holographic optical tweezers (HOT) in any direction. While these bacteria have a strong preference to align along the direction…

Phase contrast enhancement in microscopy using spiral phase filtering

Author(s): Guohai Situ, Michael Warber, Giancarlo Pedrini, Wolfgang Osten. Abstract: “We demonstrate two methods based on Fourier plane filtering using (a) a fractional spiral phase plate (SPP) and (b) an off-axial SPP for phase contrast enhancement in optical microscopy. In comparison to previous works, a spatially incoherent LED is used in the Köhler illumination as the light source to illuminate the biological specimen. We demonstrate that both these methods can transform the phase specimen into a relief-like view even under such illumination. The degree and orientation of enhancement can be controlled by changing the phase structure of the filter. The…

Self-reference quantitative phase microscopy for microfluidic devices

Author(s): Jaeduck Jang, Chae Yun Bae, Je-Kyun Park, Jong Chul Ye Abstract: “This Letter describes a quantitative phase microscopy for microfluidic devices using a simple self-referencing interferometry. Compared with the gross dimensions of the microfluidic device, the microchannel occupies only a small area of the device. Hence, the reference field can be generated by inverting the relative position of the specimen and background. Our system is realized using an extended depth-of-field optics in the form of Michelson interferometry, which allows quantitative phase measurement for an increased depth-of-field without moving objective lens or specimen. Furthermore, the system can be readily converted…

Self-reference extended depth-of-field quantitative phase microscopy

Author(s): Jaeduck Jang, Chae Yun Bae, Je-Kyun Park, and Jong Chul Ye Abstract: “This paper describes a novel quantitative phase microscopy based on a simple self-referencing scheme using Michelson interferometry. In order to achieve the homogeneous reference field for accurate phase measurement, the imaging field-of-view (FOV) is split onto the sample and homogenous background areas. The reference field can be generated by rotating the relative position of the sample and homogenous background in the object arm. Furthermore, our system is realized using an extended depth-of-field (eDOF) optics, which allows quantitative phase measurement for an increase of the depth-of-field without moving…

Plasmid copy number noise in monoclonal populations of bacteria

Author(s): Jérôme Wong Ng, Didier Chatenay, Jérôme Robert, Michael Guy Poirier Abstract: “Plasmids are extra chromosomal DNA that can confer to their hosts’ supplementary characteristics such as antibiotic resistance. Plasmids code for their copy number through their own replication frequency. Even though the biochemical networks underlying the plasmid copy number (PCN) regulation processes have been studied and modeled, no measurement of the heterogeneity in PCN within a whole population has been done. We have developed a fluorescent-based measurement system, which enables determination of the mean and noise in PCN within a monoclonal population of bacteria. Two different fluorescent protein reporters…

Nonspherical laser-induced cavitation bubbles

Author(s): Kang Yuan Lim, Pedro A. Quinto-Su, Evert Klaseboer, Boo Cheong Khoo, Vasan Venugopalan, Claus-Dieter Ohl Abstract: “The generation of arbitrarily shaped nonspherical laser-induced cavitation bubbles is demonstrated with a optical technique. The nonspherical bubbles are formed using laser intensity patterns shaped by a spatial light modulator using linear absorption inside a liquid gap with a thickness of 40 μm. In particular we demonstrate the dynamics of elliptic, toroidal, square, and V-shaped bubbles. The bubble dynamics is recorded with a high-speed camera at framing rates of up to 300 000 frames per second. The observed bubble evolution is compared to predictions from…

Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues

Author(s): Na Ji, Daniel E Milkie, Eric Betzig Abstract: “Biological specimens are rife with optical inhomogeneities that seriously degrade imaging performance under all but the most ideal conditions. Measuring and then correcting for these inhomogeneities is the province of adaptive optics. Here we introduce an approach to adaptive optics in microscopy wherein the rear pupil of an objective lens is segmented into subregions, and light is directed individually to each subregion to measure, by image shift, the deflection faced by each group of rays as they emerge from the objective and travel through the specimen toward the focus. Applying our…

Rotating single atoms in a ring lattice generated by a spatial light modulator

Author(s): Xiaodong He, Peng Xu, Jin Wang, and Mingsheng Zhan Abstract: “We demonstrated trapping single neutral Rb atoms in micro traps of an optical ring lattice formed by superposing the ±l components of the Laguerre-Gaussian mode, and generated by reflecting a single laser beam from a computer controlled spatial light modulator. A single atom in one trap or two atoms with one each in two traps were identified by observing the fluorescence. The trap array loaded with single atoms was rotated by dynamically displaying the hologram animation movie on the modulator. The modulation period in the fluorescence indicates the rotation…

Optical mirror trap with a large field of view

Author(s): Maximilian Pitzek, Ruth Steiger, Gregor Thalhammer, Stefan Bernet, and Monika Ritsch-Marte Abstract: “Holographic optical tweezers typically require microscope objectives with high numerical aperture and thus usually suffer from the disadvantage of a small field of view and a small working distance. We experimentally investigate an optical mirror trap that is created after reflection of two holographically shaped collinear beams on a mirror. This approach combines a large field of view and a large working distance with the possibility to manipulate particles in a large size range, since it allows to use a microscope objective with a numerical aperture as…

Compact Optical Tweezers Based on SLM for Real-Time Optical Trapping and Manipulation

Author(s): Martin Nyvlt, Marek Skeren Abstract: “We report a compact holographic optical tweezers based on an LCoS SLM. Optical traps are generated by diffraction of light on the Fresnel-type hologram generated by a fast parallel algorithm that enables real-time 3-D manipulation.” Link to Publications Page Publication: Conference Paper Frontiers in Optics (FiO) – via Optics InfoBase, (subscription required) Issue/Year/DOI: onference Paper Frontiers in Optics (FiO), October 11, 2009