Author(s):
Xu, Ning; Bohndiek, Sarah E.; Li, Zexing; Zhang, Cilong & Tan, Qiaofeng
Abstract:
“Point-scanning microscopy approaches are transforming super-resolution imaging. Despite achieving parallel high-speed imaging using multifocal techniques, efficient multicolor capability with high-quality illumination is currently lacking. In this paper, we present for the first time Mechanical-scan-free and multi-Color Super-resolution Microscopy (MCoSM) by spot array illumination, which enables mechanical-scan-free super-resolution
imaging with adjustable resolution and field of view (FoV) based on spatial light modulators (SLMs). Through 100s-10,000s super-resolution spot illumination with different FoV for imaging, we demonstrate the adjustable capacity of MCoSM. MCoSM extends current spectral imaging capabilities through a time-sharing process of different color illumination with phase-shifting scanning, while retaining the spatial flexibility of super-resolution imaging with diffractive spot array illumination. To showcase the prospects for further combining MCoSM with multi-color imaging, we also perform spectral unmixing (four-colors) on images of fluorescent beads at high resolution. MCoSM provides a versatile platform
for studying molecular interactions in complex samples at the nanoscale level.”
Issue/Year: arXiv, 2023
DOI: https://arxiv.org/ftp/arxiv/papers/2303/2303.06988.pdf