Nonlinear generation of Airy vortex beam

Author(s):
Hui Li and Haigang Liu and Xianfeng Chen

Abstract:

“Recently, hybrid beams have sparked considerable interest because of their properties coming from different kinds of beams at the same time. Here, we experimentally demonstrate Airy vortex beam generation in the nonlinear frequency conversion process when the fundamental wave with its phase modulated by a spatial light modulator is incident into a homogeneous nonlinear medium. In our experiments, second harmonic Airy circle vortex beams and Airy ellipse vortex beams were generated and the topological charge was also measured. The parabolic trajectory of those Airy vortex beams can be easily adjusted by altering the fundamental wave phase. This study provides a simple way to generate second harmonic Airy vortex beams, which may broaden its future use in optical manipulation and light-sheet microscopy.”

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Publication: Optics Express

Issue/Year/DOI: Optics Express Volume 26, Issue 16
DOI: 10.1364/oe.26.021204

Multiple-plane image formation by Walsh zone plates

Author(s):

Federico Machado, Vicente Ferrando, Fernando Giménez, Walter D. Furlan, and Juan A. Monsoriu

Abstract:

“A radial Walsh filter is a phase binary diffractive optical element characterized by a set of concentric rings that take the phase values 0 or π, corresponding to the values + 1 or −1 of a given radial Walsh function. Therefore, a Walsh filter can be re-interpreted as an aperiodic multifocal zone plate, capable to produce images of multiple planes simultaneously in a single output plane of an image forming system. In this paper, we experimentally demonstrate for the first time the focusing capabilities of these structures. Additionally, we report the first achievement of images of multiple-plane objects in a single image plane with these aperiodic diffractive lenses.”

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Publication: Optics Express

Issue/Year/DOI: Optics Express Volume 26, Issue 16
DOI: 10.1364/OE.26.021210

Wide-field in situ multiplexed Raman imaging with superresolution

Author(s):

Houkai Chen and Xiaojing Wu and Yuquan Zhang and Yong Yang and Changjun Min and Siwei Zhu and Xiaocong Yuan and Qiaoliang Bao and Jing Bu

Abstract:

“Because of the fingerprint-like specificity of its characteristic spectrogram, Raman spectral imaging has been applied widely in various research areas. Using a combination of structured illumination with the surfaceenhanced Raman scattering (SERS) technique, wide-field Raman imaging is developed with a significant improvement in spatial resolution. As a result of the relatively narrow Raman characteristic peaks, optically encoded SERS nanoparticles can be used to perform multiplexed imaging. The results show excellent superresolution wide-field multiplexed imaging performance. The developed technique has extraordinary potential for applications in biological imaging and other related fields.”

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Publication: Photonics Research
Issue/Year/DOI: Photonics Research Volume 6, Issue 6 pp. 530-534
DOI: 10.1364/PRJ.6.000530

Micro-Dumbbells—A Versatile Tool for Optical Tweezers

Author(s):

Weronika Lamperska, Sławomir Drobczyński, Michał Nawrot, Piotr Wasylczyk, Jan Masajada

Abstract:

“Manipulation of micro- and nano-sized objects with optical tweezers is a well-established, albeit still evolving technique. While many objects can be trapped directly with focused laser beam(s), for some applications indirect manipulation with tweezers-operated tools is preferred. We introduce a simple, versatile micro-tool operated with holographic optical tweezers. The 40 µm long dumbbell-shaped tool, fabricated with two-photon laser 3D photolithography has two beads for efficient optical trapping and a probing spike on one end. We demonstrate fluids viscosity measurements and vibration detection as examples of possible applications.”

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Publication: Micromachines

Issue/Year/DOI: Micromachines Volume 9, Issue 6
DOI: 10.3390/mi9060277

Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms

Author(s):

Liu, Tsung-Li and Upadhyayula, Srigokul and Milkie, Daniel E. and Singh, Ved and Wang, Kai and Swinburne, Ian A. and Mosaliganti, Kishore R. and Collins, Zach M. and Hiscock, Tom W. and Shea, Jamien and Kohrman, Abraham Q. and Medwig, Taylor N. and Dambournet, Daphne and Forster, Ryan and Cunniff, Brian and Ruan, Yuan and Yashiro, Hanako and Scholpp, Steffen and Meyerowitz, Elliot M. and Hockemeyer, Dirk and Drubin, David G. and Martin, Benjamin L. and Matus, David Q. and Koyama, Minoru and Megason, Sean G. and Kirchhausen, Tom and Betzig, Eric

Abstract:

“True physiological imaging of subcellular dynamics requires studying cells within their parent organisms, where all the environmental cues that drive gene expression, and hence the phenotypes that we actually observe, are present. A complete understanding also requires volumetric imaging of the cell and its surroundings at high spatiotemporal resolution, without inducing undue stress on either. We combined lattice light-sheet microscopy with adaptive optics to achieve, across large multicellular volumes, noninvasive aberration-free imaging of subcellular processes, including endocytosis, organelle remodeling during mitosis, and the migration of axons, immune cells, and metastatic cancer cells in vivo. The technology reveals the phenotypic diversity within cells across different organisms and developmental stages and may offer insights into how cells harness their intrinsic variability to adapt to different physiological environments.”

 

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Publication: Science

Issue/Year/DOI: Science, Vol. 360, Issue 6386, (2018)
DOI: 10.1126/science.aaq1392

Single shot, three-dimensional fluorescence microscopy with a spatially rotating point spread function

Author(s):

Zhaojun Wang and Yanan Cai and Yansheng Liang and Xing Zhou and Shaohui Yan and Dan Dan and Piero R. Bianco and Ming Lei and Baoli Yao

Abstract:

“A wide-field fluorescence microscope with a double-helix point spread function (PSF) is constructed to obtain the specimen’s three-dimensional distribution with a single snapshot. Spiral-phase-based computer-generated holograms (CGHs) are adopted to make the depth-of-field of the microscope adjustable. The impact of system aberrations on the double-helix PSF at high numerical aperture is analyzed to reveal the necessity of the aberration correction. A modified cepstrum-based reconstruction scheme is promoted in accordance with properties of the new double-helix PSF. The extended depth-of-field images and the corresponding depth maps for both a simulated sample and a tilted section slice of bovine pulmonary artery endothelial (BPAE) cells are recovered, respectively, verifying that the depth-of-field is properly extended and the depth of the specimen can be estimated at a precision of 23.4nm. This three-dimensional fluorescence microscope with a framerate-rank time resolution is suitable for studying the fast developing process of thin and sparsely distributed micron-scale cells in extended depth-of-field.”

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Publication: Biomedical Optics Express

Issue/Year/DOI: Biomedical Optics Express, Vol. 8, Issue 12, (2017)
DOI: 10.1364/BOE.8.005493

Binary phase masks for easy system alignment and basic aberration sensing with spatial light modulators in STED microscopy

Author(s):

Klauss, André and Conrad, Florian and Hille, Carsten Hille

Abstract:

“The use of binary phase patterns to improve the integration and optimization of spatial light modulators (SLM) in an imaging system, especially a confocal microscope, is proposed and demonstrated. The phase masks were designed to create point spread functions (PSF), which exhibit specific sensitivity to major disturbances in the optical system. This allows direct evaluation of misalignment and fundamental aberration modes by simple visual inspection of the focal intensity distribution or by monitoring the central intensity of the PSF. The use of proposed phase masks is investigated in mathematical modelling and experiment for the use in a stimulated emission depletion (STED) microscope applying wavefront shaping by a SLM. We demonstrate the applicability of these phase masks for modal wavefront sensing of low order aberration modes up to the third order of Zernike polynomials, utilizing the point detector of a confocal microscope in a ‘guide star’ approach. A lateral resolution of ~25 nm is shown in STED imaging of the confocal microscope retrofitted with a SLM and a STED laser and binary phase mask based system optimization.”

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Publication: Scientific Reports
Issue/Year/DOI: Scientific Reports Volume 7, Article number: 15699 (2017)
DOI: 10.1038/s41598-017-15967-5

Silver Nanowires for Reconfigurable Bloch Surface Waves.

Author(s):

Zhang, Douguo and Wang, Ruxue and Xiang, Yifeng and Kuai, Yan and Kuang, Cuifang and Badugu, Ramachandram and Xu, Yingke and Wang, Pei and Ming, Hai and Liu, Xu and Lakowicz, Joseph R.

Abstract:

“The use of a single silver nanowire as a flexible coupler to transform a free space beam into a Bloch surface wave propagating on a dielectric multilayer is proposed. Based on Huygens ‘Principle, when a Gaussian beam is focused onto a straight silver nanowire, a Bloch surface wave is generated and propagates perpendicular to the nanowire. By curving the silver nanowire, the surface wave can be focused. Furthermore, the spatial phase of the incident laser beam can be actively controlled with the aid of a spatial light modulator, resulting in the reconfigurable or dynamically controlled Bloch surface waves. The low cost of the chemically synthesized silver nanowires and the high flexibility with regard to tuning the spatial phase of the incident light make this approach very promising for various applications including optical micromanipulation, fluorescence imaging, and sensing.”

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Publication: ACS Nano

Issue/Year/DOI:  ACS Nano, 2017, 11 (10), pp 10446–10451
DOI: 10.1021/acsnano.7b05638

Precise spatio-temporal control of rapid optogenetic cell ablation with mem-KillerRed in Zebrafish.

Author(s):

Buckley, C. and Carvalho, M. T. and Young, L. K. and Rider, S. A. and McFadden, C. and Berlage, C. and Verdon, R. F. and Taylor, J. M. and Girkin, J. M. and Mullins, J. J.

Abstract:

“The ability to kill individual or groups of cells in vivo is important for studying cellular processes and their physiological function. Cell-specific genetically encoded photosensitizing proteins, such as KillerRed, permit spatiotemporal optogenetic ablation with low-power laser light. We report dramatically improved resolution and speed of cell targeting in the zebrafish kidney through the use of a selective plane illumination microscope (SPIM). Furthermore, through the novel incorporation of a Bessel beam into the SPIM imaging arm, we were able to improve on targeting speed and precision. The low diffraction of the Bessel beam coupled with the ability to tightly focus it through a high NA lens allowed precise, rapid targeting of subsets of cells at anatomical depth in live, developing zebrafish kidneys. We demonstrate that these specific targeting strategies significantly increase the speed of optoablation as well as fish survival.”

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Publication: Scientific Reports

Issue/Year/DOI: Scientific Reports, volume 7, Article number: 5096 (2017)
DOI: 10.1038/s41598-017-05028-2

Coherent optical adaptive technique improves the spatial resolution of STED microscopy in thick samples

Author(s):

Wei Yan and Yanlong Yang and Yu Tan and Xun Chen and Yang Li and Junle Qu and Tong Ye

Abstract:

“Stimulated emission depletion (STED) microscopy is one of far-field optical microscopy techniques that can provide sub-diffraction spatial resolution. The spatial resolution of the STED microscopy is determined by the specially engineered beam profile of the depletion beam and its power. However, the beam profile of the depletion beam may be distorted due to aberrations of optical systems and inhomogeneity of a specimen’s optical properties, resulting in a compromised spatial resolution. The situation gets deteriorated when thick samples are imaged. In the worst case, the severe distortion of the depletion beam profile may cause complete loss of the super-resolution effect no matter how much depletion power is applied to specimens. Previously several adaptive optics approaches have been explored to compensate aberrations of systems and specimens. However, it is difficult to correct the complicated high-order optical aberrations of specimens. In this report, we demonstrate that the complicated distorted wavefront from a thick phantom sample can be measured by using the coherent optical adaptive technique. The full correction can effectively maintain and improve spatial resolution in imaging thick samples.”

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Publication: Photonics Research

Issue/Year/DOI: Photonics Research, Vol. 5, Issue3, pp. 176-181 (2017)
DOI: 10.1364/PRJ.5.000176

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