Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms

Author(s):

Liu, Tsung-Li and Upadhyayula, Srigokul and Milkie, Daniel E. and Singh, Ved and Wang, Kai and Swinburne, Ian A. and Mosaliganti, Kishore R. and Collins, Zach M. and Hiscock, Tom W. and Shea, Jamien and Kohrman, Abraham Q. and Medwig, Taylor N. and Dambournet, Daphne and Forster, Ryan and Cunniff, Brian and Ruan, Yuan and Yashiro, Hanako and Scholpp, Steffen and Meyerowitz, Elliot M. and Hockemeyer, Dirk and Drubin, David G. and Martin, Benjamin L. and Matus, David Q. and Koyama, Minoru and Megason, Sean G. and Kirchhausen, Tom and Betzig, Eric

Abstract:

“True physiological imaging of subcellular dynamics requires studying cells within their parent organisms, where all the environmental cues that drive gene expression, and hence the phenotypes that we actually observe, are present. A complete understanding also requires volumetric imaging of the cell and its surroundings at high spatiotemporal resolution, without inducing undue stress on either. We combined lattice light-sheet microscopy with adaptive optics to achieve, across large multicellular volumes, noninvasive aberration-free imaging of subcellular processes, including endocytosis, organelle remodeling during mitosis, and the migration of axons, immune cells, and metastatic cancer cells in vivo. The technology reveals the phenotypic diversity within cells across different organisms and developmental stages and may offer insights into how cells harness their intrinsic variability to adapt to different physiological environments.”

 

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Publication: Science

Issue/Year/DOI: Science, Vol. 360, Issue 6386, (2018)
DOI: 10.1126/science.aaq1392

Generation of optical vortex array along arbitrary curvilinear arrangement

Author(s):

Lin Li and Chenliang Chang and Xiangzheng Yuan and Caojin Yuan and Shaotong Feng and Shouping Nie and Jianping Ding

Abstract:

“We propose an approach for creating optical vortex array (OVA) arranged along arbitrary curvilinear path, based on the coaxial interference of two width-controllable component curves calculated by modified holographic beam shaping technique. The two component curve beams have different radial dimensions as well as phase gradients along each beam such that the number of phase singularity in the curvilinear arranged optical vortex array (CA-OVA) is freely tunable on demand. Hybrid CA-OVA that comprises of multiple OVA structures along different respective curves is also discussed and demonstrated. Furthermore, we study the conversion of CA-OVA into vector mode that comprises of polarization vortex array with varied polarization state distribution. Both simulation and experimental results prove the performance of the proposed method of generating a complex structured vortex array, which is of significance for potential applications including multiple trapping of micro-sized particles.”

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Publication: Optics Express

Issue/Year/DOI: Optics Express, Vol.26, Issue 8, pp. 9798- 9812 (2018)
DOI: 10.1364/OE.26.009798

Spin-orbit interaction of light induced by transverse spin angular momentum engineering

Author(s):

Zengkai Shao and Jiangbo Zhu and Yujie Chen and Yanfeng Zhang and Siyuan Yu

Abstract:

“The investigations on optical angular momenta and their interactions have broadened our knowledge of light’s behavior at sub-wavelength scales. Recent studies further unveil the extraordinary characteristics of transverse spin angular momentum in confined light fields and orbital angular momentum in optical vortices. Here we demonstrate a direct interaction between these two intrinsic quantities of light. By engineering the transverse spin in the evanescent wave of a whispering-gallery-mode-based optical vortex emitter, a spin-orbit interaction is observed in generated vortex beams. Inversely, this unconventional spin-orbit interplay further gives rise to an enhanced spin-direction locking effect in which waveguide modes are unidirectionally excited, with the directionality jointly controlled by the spin and orbital angular momenta states of light. The identification of this previously unknown pathway between the polarization and spatial degrees of freedom of light enriches the spin-orbit interaction phenomena, and can enable various functionalities in applications such as communications and quantum information processing.”

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Publication: Nature Communications

Issue/Year/DOI: Nature Communicationsvolume 9, Article number: 926 (2018)
DOI: 10.1038/s41467-018-03237-5

Synthesis of light needles with tunable length and nearly constant irradiance.

Author(s):

Martínez-Herrero, Rosario and Maluenda, David and Juvells, Ignasi and Carnicer, Artur

Abstract:

“We introduce a new method for producing optical needles with tunable length and almost constant irradiance based on the evaluation of the on-axis power content of the light distribution at the focal area. According to theoretical considerations, we propose an adaptive modulating continuous function that presents a large derivative and a zero value jump at the entrance pupil of the focusing system. This distribution is displayed on liquid crystal devices using holographic techniques. In this way, a polarized input beam is shaped and subsequently focused using a high numerical aperture (NA) objective lens. As a result, needles with variable length and nearly constant irradiance are produced using conventional optics components. This procedure is experimentally demonstrated obtaining a 53λ-long and 0.8λ-wide needle.”

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Publication: Scientific Reports

Issue/Year/DOI: Scientific Reportsvolume 8, Article number: 2657 (2018)
DOI: 10.1038/s41598-018-21007-7

Controllable mode transformation in perfect optical vortices

Author(s):

Xinzhong Li and Haixiang Ma and Chuanlei Yin and Jie Tang and Hehe Li and Miaomiao Tang and Jingge Wang and Yuping Tai and Xiufang Li and Yishan Wang

Abstract:

“We report a novel method to freely transform the modes of a perfect optical vortex (POV). By adjusting the scaling factor of the Bessel–Gauss beam at the object plane, the POV mode transformation can be easily controlled from circle to ellipse with a high mode purity. Combined with the modulation of the cone angle of an axicon, the ellipse mode can be freely adjusted along the two orthogonal directions. The properties of the “perfect vortex” are experimentally verified. Moreover, fractional elliptic POVs with versatile modes are presented, where the number and position of the gaps are controllable. These findings are significant for applications that require the complex structured optical field of the POV.”

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Publication: Opt. Express

Issue/Year/DOI: Opt. Express, Vol. 26, Issue 2, pp. 651-662 (2018)
DOI: 10.1364/OE.26.000651

Tunable third harmonic generation of vortex beams in an optical superlattice

Author(s):

Yu Wu and Rui Ni and Zhou Xu and Yaodong Wu and Xinyuan Fang and Dan Wei and Xiaopeng Hu and Yong Zhang and Min Xiao and Shining Zhu

Abstract:

“We report generation of tunable vortex beams in the blue spectral range, with a 3.3 nm spectral tuning range, by frequency tripling of the near-infrared (IR) wave at around 1.34 um in a LiTaO3 optical superlattice. The nonlinear crystal used in this work has a chirped dual-periodical structure which can provide two expanded reciprocal vectors for tunable performance of the cascaded third harmonic generation (THG). The maximum THG efficiency reaches about 1.4%.”

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Publication: Optics Express

Issue/Year/DOI: Optics Express, Vol. 25, Issue 25, pp. 30820- 30826 (2017)
DOI: 10.1364/OE.25.030820

Dynamic three-dimensional multifocal spots in high numerical-aperture objectives

Author(s):

Linwei Zhu and Rui Yang and Dawei Zhang and Junjie Yu and Jiannong Chen

Abstract:

“Multifocal spots in high numerical-aperture (NA) objectives has emerged as a rapid, parallel, and multi-location method in a multitude of applications. However, the typical method used for forming three-dimensional (3D) multifocal spots based on iterative algorithms limits the potential applications. We demonstrate a non-iterative method using annular subzone phases (ASPs) that are composed of many annular subareas in which phase-only distributions with different 3D displacements are filled. The dynamic 3D multifocal spots with controllable position of each focal spot in the focal volume of the objective are created using the ASPs. The experimental results of such dynamic tunable 3D multifocal spots offer the possibility of versatile process in laser 3D fabrication, optical trapping, and fast focusing scanned microscopic imaging.”

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Publication: Optics Express

Issue/Year/DOI: Optics Express, Vol. 25, Issue 20, pp. 24756- 24766 (2018)
DOI: 10.1364/OE.25.024756

Real and virtual propagation dynamics of angular accelerating white light beams

Author(s):

Christian Vetter and Angela Dudley and Alexander Szameit and Andrew Forbes

Abstract:

“Accelerating waves have received significant attention of late, first in the optical domain and later in the form of electron matter waves, and have found numerous applications in non-linear optics, material processing, microscopy, particle manipulation and laser plasma interactions. Here we create angular accelerating light beams with a potentially unlimited acceleration rate. By employing wavelength independent digital holograms for the creation and propagation of white light beams, we are able to study the resulting propagation in real and virtual space. We find that dephasing occurs for real propagation and that this can be compensated for in a virtual propagation scheme when single plane dynamics are important. Our work offers new insights into the propagation dynamics of such beams and provides a versatile tool for further investigations into propagating structured light fields.”

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Publication: Optics Express

Issue/Year/DOI: Optics Express Vol. 25, Issue 17, pp. 20530-20540 (2017)

DOI: 10.1364/OE.25.020530

 

Non-diffractive Bessel-Gauss beams for the detection of rotating object free of obstructions

Author(s):

Shiyao Fu and Tonglu Wang and Zheyuan Zhang and Yanwang Zhai and Chunqing Gao

Abstract:

“Bessel-Gauss beams carrying orbital angular momentum are widely known for their non-diffractive or self-reconstructing performance, and have been applied in lots of domains. Here we demonstrate that, by illuminating a rotating object with high-order Bessel-Gauss beams, a frequency shift proportional to the rotating speed and the topological charge is observed. Moreover, the frequency shift is still present once an obstacle exists in the path, in spite of the decreasing of received signals. Our work indicates the feasibility of detecting rotating objects free of obstructions, and has potential as obstruction-immune rotation sensors in engine monitoring, aerological sounding, and so on.”

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Publication: Optics Express

Issue/Year/DOI: Optics Express , Vol. 25, Issue 17, pp. 20098- 20108 (2018)
DOI: 10.1364/OE.25.020098

Axial sub-Fourier focusing of an optical beam

Author(s):

Thomas Zacharias and Barak Hadad and Alon Bahabad and Yaniv Eliezer

Abstract:

“We demonstrate experimentally the generation of an optical beam having an axial focusing that is narrower than the Fourier limit. The beam is constructed from a superposition of Bessel beams with different longitudinal wave vectors, realizing a super-oscillatory axial intensity distribution. Such beams can be useful for microscopy and for optical particle manipulation.”

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Publication: Optics Letters

Issue/Year/DOI: Optics Letters Vol. 42, Issue 16, pp. 3205-3208 (2017)

DOI: 10.1364/OL.42.003205