Combination of scene-based and stochastic measurement for wide-field aberration correction in microscopic imaging

Author(s):

Michael Warber, Selim Maier, Tobias Haist, and Wolfgang Osten

Abstract:

“We report on a novel aberration correction technique that uses the sequential combination of two different aberration measurement methods to correct for setup-induced and specimen-induced aberrations. The advantages of both methods are combined and, thus, the measurement time is strongly reduced without loss of accuracy. The technique is implemented using a spatial-light-modulator-based wide-field microscope without the need for additional components (e.g., a Shack–Hartmann sensor). The aberrations are measured without a reference object by directly using the specimen to be imaged. We demonstrate experimental results for technical as well as biological specimens.”

Link to Publications Page

Publication: Applied Optics

Issue/Year/DOI: Vol. 49, Issue 28, pp. 5474-5479 (2010)
DOI: 10.1364/AO.49.005474