Author(s): Liisa M. Hirvonen, Kai Wicker, Ondrej Mandula und Rainer Heintzmann

Abstract:

“Due to diffraction, the resolution of imaging emitted light in a fluorescence microscope is limited to about 200 nm in the lateral direction. Resolution improvement by a factor of two can be achieved using structured illumination, where a fine grating is projected onto the sample, and the final image is reconstructed from a set of images taken at different grating positions. Here we demonstrate that with the help of a spatial light modulator, this technique can be used for imaging slowly moving structures in living cells.”

Link to Publications Page

Publication: European Biophysics Journal, (free download)

Issue/Year/DOI: European Biophysics Journal, Volume 38, Number 6, 807-812 (2009)
doi:10.1007/s00249-009-0501-6