Author(s): Reto Fiolka, Markus Beck, and Andreas Stemmer

Abstract:

“In wide-field fluorescence microscopy, illuminating the specimen with evanescent standing waves increases lateral resolution more than twofold. We report a versatile setup for standing-wave illumination in total internal reflection fluorescence microscopy. An adjustable diffraction grating written on a phase-only spatial light modulator controls the illumination field. Selecting appropriate diffraction orders and displaying a sheared (tilted) diffraction grating allows one to tune the penetration depth in very fine steps. The setup achieves 91 nm lateral resolution for green emission.”

Link to Publications Page

Publication: Optics Letters (subscription required)

Issue/Year/DOI: Optics Letters, Vol. 33, Issue 14, pp. 1629-1631
doi:10.1364/OL.33.001629