Author(s): Stefan Bernet, Alexander Jesacher, Severin Fürhapter, Christian Maurer, and Monika Ritsch-Marte

Abstract:

“Recently a spatial spiral phase filter in a Fourier plane of a microscopic imaging setup has been demonstrated to produce edge enhancement and relief-like shadow formation of amplitude and phase samples. Here we demonstrate that a sequence of at least 3 spatially filtered images, which are recorded with different rotational orientations of the spiral phase plate, can be used to obtain a quantitative reconstruction of both, amplitude and phase information of a complex microscopic sample, i.e. an object consisting of mixed absorptive and refractive components. The method is demonstrated using a calibrated phase sample, and an epithelial cheek cell.”

Link to Publications Page

Publication: Optics Express (free download)

Issue/Year/DOI: Optics Express, Vol. 14, Issue 9, pp. 3792-3805
doi:10.1364/OE.14.003792