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Interference patterns of two-photon excited fluorescence by spatial beam shaping

PLUTO / PLUTO-2 Spatial Light Modulators
Adaptive Optics / Wavefront Control Imaging/ Image Processing Microscopy
Published on:
Authors: R. Gottschalk, A. Lindinger
Abstract:

We report on two-photon excited fluorescence interference patterns produced by spatial laser beam shaping. Thereto, a focused spatially modulated laser beam component is overlapped with an unfocused Gaussian beam component, and both are directed on a cuvette filled with dye. The phase retardance between the two common path laser components is precisely adjusted by a 2D liquid crystal modulator. With this method it is possible to generate various fluorescence patterns which exhibit sharp features and a high contrast due to constructive and destructive interference of a two-photon excitation process. The developed spatial shaping interference technique with two-photon excitations has a high potential for optical and biophotonic imaging applications.

Open Access

Open Access

Publication: Optik
Issue/Year: Optik, Volume 316, 2024, 172054
DOI: 10.1016/j.ijleo.2024.172054
Link: https://doi.org/10.1016/j.ijleo.2024.172054
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