Author(s): Alexander Jesacher, Severin Fürhapter, Stefan Bernet, and Monika Ritsch-Marte


“We demonstrate a flexible setup for holographic steering of laser tweezers in microscopy using a high resolution spatial light modulator (SLM). In contrast to other methods, hologram read-out is done in the off-axis Fresnel regime rather than in the typically used on-axis Fourier regime. The diffractive structure is calculated as a Fresnel hologram, such that after reflection at the SLM only the desired first diffraction order is guided to the input of an optical microscope, where it generates a tailored optical tweezers field. We demonstrate some advantageous features of this setup, i.e. undesired diffraction orders are suppressed, the optical traps can be easily steered in real-time by just “mouse-dragging” a hologram window at the SLM display, and a number of independently steerable optical traps can be generated simultaneously in a three-dimensional arrangement by displaying a corresponding number of adjacent hologram windows at the SLM screen.”

Link to Publications Page

Publication: Optics Express
Issue/Year: Optics Express, Vol. 12, Issue 10, pp. 2243-2250, 2004
DOI: 10.1364/OPEX.12.002243