Singularities splitting phenomenon for the superposition of hybrid orders structured lights and the corresponding interference discrimination method

Author(s):

Mao, Baiwei; Liu, Yange; Chang, Wenzhe; Chen, Liang; Feng, Mao; Guo, Huiyi; He, Jiangyong & Wang, Zhi

Abstract:

“It is the basic characteristic of pure vortex light

that there is a phase singularity at the origin. Such a sin-

gularity may be multiple degenerate, which determines the

order of vortex light. Singularities splitting phenomenon

means that singularities no longer concentrate at the origin

but distribute around the space, usually occurring in

impure vortex light. In this paper, we demonstrate the

singularities splitting phenomenon and propose an anal-

ysis method, based on which one may rapidly estimate the

modal components of impure vortex light. As two common

singularity discrimination methods, the spiral and fork

wire interference patterns are compared in distinguishing

splitting singularities. The most widely used spiral inter-

ference pattern is revealed to be the worst form because of

the low resolution. Instead, the fork wire interference

pattern is with higher and easily adjusted resolution. 1‰

impurity is still able to be distinguished through fork wire

interference patterns in the experiment.”

Link to Publications Page

Publication: Nanophotonics
Issue/Year: Nanophotonics, Volume 0; Number 0; 2022
DOI: 10.1515/nanoph-2021-0814

Large depth-of-field fluorescence microscopy based on deep learning supported by Fresnel incoherent correlation holography

Author(s):

Wu, Peng; Zhang, Dejie; Yuan, Jing; Zeng, Shaoqun; Gong, Hui; Luo, Qingming & Yang, Xiaoquan

Abstract:

“Fluorescence microscopy plays an irreplaceable role in biomedicine. However,

limited depth of field (DoF) of fluorescence microscopy is always an obstacle of image quality,

especially when the sample is with an uneven surface or distributed in different depths. In

this manuscript, we combine deep learning with Fresnel incoherent correlation holography to

describe a method to obtain significant large DoF fluorescence microscopy. Firstly, the hologram

is restored by the Auto-ASP method from out-of-focus to in-focus in double-spherical wave

Fresnel incoherent correlation holography. Then, we use a generative adversarial network to

eliminate the artifacts introduced by Auto-ASP and output the high-quality image as a result.

We use fluorescent beads, USAF target and mouse brain as samples to demonstrate the large

DoF of more than 400µm, which is 13 times better than that of traditional wide-field microscopy.

Moreover, our method is with a simple structure, which can be easily combined with many

existing fluorescence microscopic imaging technology”

Link to Publications Page

Publication: Optics Express
Issue/Year: Optics Express, Volume 30; Number 4; Pages 5177; 2022
DOI: 10.1364/oe.451409