Demonstration of a vectorial optical field generator with adaptive close loop control

Author(s):

Jian Chen and Lingjiang Kong and Qiwen Zhan

Abstract:

“We experimentally demonstrate a vectorial optical field generator (VOF-Gen) with an adaptive close loop control. The close loop control capability is illustrated with the calibration of polarization modulation of the system. To calibrate the polarization ratio modulation, we generate 45° linearly polarized beam and make it propagate through a linear analyzer whose transmission axis is orthogonal to the incident beam. For the retardation calibration, circularly polarized beam is employed and a circular polarization analyzer with the opposite chirality is placed in front of the CCD as the detector. In both cases, the close loop control automatically changes the value of the corresponding calibration parameters in the pre-set ranges to generate the phase patterns applied to the spatial light modulators and records the intensity distribution of the output beam by the CCD camera. The optimized calibration parameters are determined corresponding to the minimum total intensity in each case. Several typical kinds of vectorial optical beams are created with and without the obtained calibration parameters, and the full Stokes parameter measurements are carried out to quantitatively analyze the polarization distribution of the generated beams. The comparisons among these results clearly show that the obtained calibration parameters could remarkably improve the accuracy of the polarization modulation of the VOF-Gen, especially for generating elliptically polarized beam with large ellipticity, indicating the significance of the presented close loop in enhancing the performance of the VOF-Gen.”

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Publication: Review of Scientific Instruments

Issue/Year/DOI: Review of SCientific Instruments 88, 125111 (2017)
DOI: 10.1063/1.4999656

Tunable third harmonic generation of vortex beams in an optical superlattice

Author(s):

Yu Wu and Rui Ni and Zhou Xu and Yaodong Wu and Xinyuan Fang and Dan Wei and Xiaopeng Hu and Yong Zhang and Min Xiao and Shining Zhu

Abstract:

“We report generation of tunable vortex beams in the blue spectral range, with a 3.3 nm spectral tuning range, by frequency tripling of the near-infrared (IR) wave at around 1.34 um in a LiTaO3 optical superlattice. The nonlinear crystal used in this work has a chirped dual-periodical structure which can provide two expanded reciprocal vectors for tunable performance of the cascaded third harmonic generation (THG). The maximum THG efficiency reaches about 1.4%.”

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Publication: Optics Express

Issue/Year/DOI: Optics Express, Vol. 25, Issue 25, pp. 30820- 30826 (2017)
DOI: 10.1364/OE.25.030820

Single shot, three-dimensional fluorescence microscopy with a spatially rotating point spread function

Author(s):

Zhaojun Wang and Yanan Cai and Yansheng Liang and Xing Zhou and Shaohui Yan and Dan Dan and Piero R. Bianco and Ming Lei and Baoli Yao

Abstract:

“A wide-field fluorescence microscope with a double-helix point spread function (PSF) is constructed to obtain the specimen’s three-dimensional distribution with a single snapshot. Spiral-phase-based computer-generated holograms (CGHs) are adopted to make the depth-of-field of the microscope adjustable. The impact of system aberrations on the double-helix PSF at high numerical aperture is analyzed to reveal the necessity of the aberration correction. A modified cepstrum-based reconstruction scheme is promoted in accordance with properties of the new double-helix PSF. The extended depth-of-field images and the corresponding depth maps for both a simulated sample and a tilted section slice of bovine pulmonary artery endothelial (BPAE) cells are recovered, respectively, verifying that the depth-of-field is properly extended and the depth of the specimen can be estimated at a precision of 23.4nm. This three-dimensional fluorescence microscope with a framerate-rank time resolution is suitable for studying the fast developing process of thin and sparsely distributed micron-scale cells in extended depth-of-field.”

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Publication: Biomedical Optics Express

Issue/Year/DOI: Biomedical Optics Express, Vol. 8, Issue 12, (2017)
DOI: 10.1364/BOE.8.005493

Fast label-free microscopy technique for 3D dynamic quantitative imaging of living cells

Author(s):

José A. Rodrigo, Juan M. Soto, and Tatiana Alieva

Abstract:

“The refractive index (RI) is an important optical characteristic that is often exploited in label-free microscopy for analysis of biological objects. A technique for 3D RI reconstruction of living cells has to be fast enough to capture the cell dynamics and preferably needs to be compatible with standard wide-field microscopes. To solve this challenging problem, we present a technique that provides fast measurement and processing of data required for real-time 3D visualization of the object RI. Specifically, the 3D RI is reconstructed from the measurement of bright-field intensity images, axially scanned by a high-speed focus tunable lens mounted in front of a sCMOS camera, by using a direct deconvolution approach designed for partially coherent light microscopy in the non-paraxial regime. Both the measurement system and the partially coherent illumination, that provides optical sectioning and speckle-noise suppression, enable compatibility with wide-field microscopes resulting in a competitive and affordable alternative to the current holographic laser microscopes. Our experimental demonstrations show video-rate 3D RI visualization of living bacteria both freely swimming and optically manipulated by using freestyle laser traps allowing for their trapping and transport along 3D trajectories. These results prove that is possible to conduct simultaneous 4D label-free quantitative imaging and optical manipulation of living cells, which is promising for the study of the cell biophysics and biology.”

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Publication: Optics Express

Issue/Year/DOI: Optics Express Volume 8, Issue 12
DOI: 10.1364/BOE.8.005507