Vertical differential interference contrast

Author(s): Michael Warber, Tobias Haist, Malte Hasler, and Wolfgang Osten


“We propose a new phase contrast filtering technique based on a combination of a focused and a defocused point-spread-function. This way, an axial shear is introduced in the imaging system. Compared to conventional differential interference contrast, an isotropic behavior is achieved. The lateral resolution is improved compared to conventional defocusing. Furthermore, the digital combination of multiple images leads to strongly enhanced visualization of small structures. We show simulated results as well as experimental results using a spatial-light modulator-based microscope.”

Link to Publications Page

Publication: Optical Engineering, (subscription required)

Issue/Year/DOI: Opt. Eng., Volume 51, Issue 1, 013204, (2012)