Structured illumination in total internal reflection fluorescence microscopy using a spatial light modulator

Author(s): Reto Fiolka, Markus Beck, and Andreas Stemmer

Abstract:

“In wide-field fluorescence microscopy, illuminating the specimen with evanescent standing waves increases lateral resolution more than twofold. We report a versatile setup for standing-wave illumination in total internal reflection fluorescence microscopy. An adjustable diffraction grating written on a phase-only spatial light modulator controls the illumination field. Selecting appropriate diffraction orders and displaying a sheared (tilted) diffraction grating allows one to tune the penetration depth in very fine steps. The setup achieves 91 nm lateral resolution for green emission.”

Link to Publications Page

Publication: Optics Letters
Issue/Year: Optics Letters, Vol. 33, Issue 14, pp. 1629-1631
DOI: 10.1364/OL.33.001629

Phase dynamics of continuous topological upconversion in vortex beams

Author(s): Carlos López-Mariscal, Daniel Burnham, Daniel Rudd, David McGloin, and Julio C. Gutiérrez-Vega

Abstract:

“The vortex emergence process as an integer order Bessel field progresses continuously onto the contiguous higher order Bessel field is studied in detail. We assess the progressive migration of phase singularities and explain the predicted increase in fractional orbital angular momentum content of the beam in terms of this gradual process.”

Link to Publications Page

Publication: Optics Express
Issue/Year: Optics Express, Vol. 16, Issue 15, pp. 11411-11422, (2008)
DOI: 10.1364/OE.16.011411

Prediction of phase-mostly modulation for holographic optical tweezers

Author(s): J. Andilla , E. Martín-Badosa, S. Vallmitjana

Abstract:

“We characterize a reflective Holoeye LC-R 2500 spatial light modulator with a technique in which Jones matrices describing its polarization capabilities are obtained and then used for any arbitrary configuration. We apply this method to predict a phase-mostly modulation response with minimum amplitude contrast and a phase modulation range close to 2π rad. This allows us to generate multiple traps in a holographic optical tweezers setup with high light efficiency and hardly any unwanted energy on the zero diffraction order.”

Link to Publications Page

Publication: Optics Communications
Issue/Year: Optics Communications, Volume 281, Issue 14, 15 July 2008, Pages 3786-3791
DOI: 10.1016/j.optcom.2008.03.067