Fluorescence endomicroscopy with structured illumination

Author(s): Nenad Bozinovic, Cathie Ventalon, Tim Ford, and Jerome Mertz


“We present an endomicroscope apparatus that utilizes structured illumination to produce high resolution (~ 2.6µm) optically sectioned fluorescence images over a field of view of about 240µm. The endomicroscope is based on the use of a flexible imaging fiber bundle with a miniaturized objective. We also present a strategy to largely suppress structured illumination artifacts that arise when imaging in thick tissue that exhibits significant out-of-focus background. To establish the potential of our endomicroscope for preclinical or clinical applications, we provide images of BCECF-AM labeled rat colonic mucosa.”

Link to Publications Page

Publication: Optics Express
Issue/Year: Optics Express, Vol. 16, Issue 11, pp. 8016-8025
DOI: 10.1364/OE.16.008016