Fluorescence endomicroscopy with structured illumination

May, 2008

Author(s): Nenad Bozinovic, Cathie Ventalon, Tim Ford, and Jerome Mertz

Abstract:

“We present an endomicroscope apparatus that utilizes structured illumination to produce high resolution (~ 2.6µm) optically sectioned fluorescence images over a field of view of about 240µm. The endomicroscope is based on the use of a flexible imaging fiber bundle with a miniaturized objective. We also present a strategy to largely suppress structured illumination artifacts that arise when imaging in thick tissue that exhibits significant out-of-focus background. To establish the potential of our endomicroscope for preclinical or clinical applications, we provide images of BCECF-AM labeled rat colonic mucosa.”

Link to Publications Page

Publication: Optics Express (free download)

Issue/Year/DOI: Optics Express, Vol. 16, Issue 11, pp. 8016-8025
doi:10.1364/OE.16.008016


Dynamic speckle illumination microscopy with wavelet prefiltering

June, 2007

Author(s): Cathie Ventalon, Rainer Heintzmann, and Jerome Mertz

Abstract:

“Dynamic speckle illumination (DSI) provides a simple and robust technique to obtain fluorescence depth sectioning with a widefield microscope. We report a significant improvement to DSI microscopy based on a statistical image-processing algorithm that incorporates spatial wavelet prefiltering. The resultant gain in sectioning strength leads to a fundamentally improved scaling law for the out-of-focus background rejection.”

Link to Publications Page

Publication: Optics Letters, (subscription required)

Issue/Year/DOI: Optics Letters, Vol. 32, Issue 11, pp. 1417-1419
doi:10.1364/OL.32.001417


Dynamic speckle illumination microscopy with translated versus randomized speckle patterns

August, 2006

Author(s): Cathie Ventalon and Jerome Mertz

Abstract:

“Dynamic speckle illumination (DSI) microscopy is a widefield fluorescence imaging technique that provides depth discrimination. The technique relies on the illumination of a sample with a sequence of speckle patterns. We consider an image processing algorithm based on a differential intensity variance between consecutive images, and demonstrate that DSI sectioning strength depends on the dynamics of the speckle pattern. Translated speckle patterns confer greater sectioning strength than randomized speckle patterns because they retain out-of-focus correlations that lead to better background rejection. We present a theory valid for arbitrary point-spread-functions, which we corroborate with experimental results.”

Link to Publications Page

Publication: Optics Express (free download)

Issue/Year/DOI: Optics Express, Vol. 14, Issue 16, pp. 7198-7209
doi:10.1364/OE.14.007198